Histone H2B Acetyl (K5) ELISA Kit antibody RabMAb®

Cat.#: 6110-1
Abcam ID: ab138916

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Bulk and Custom formulation available Email request

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A Simple, Complete ELISA Kit

- All-In-One: Quick and easy sandwich ELISA Kit
- Sensitive: Useful for detection of Histone H2B when acetylated at K5

Description

Changes in chromatin structure play a key role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Histones function as a structural component in the nucleus and as a regulator of the gene expression. Acetylation of core histones regulates gene expression (2). Histone H2B is acetylated in the N-terminal region, primary at lysines 5, 12, 15, and 20 (3). Ubiquitylation of histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of cell apoptosis and meiosis (5). Histone modifications promote or prevent binding of proteins that drive particular region of the genome into active transcription or repression. This assay employs the sandwich enzyme immunoassay technique that detects endogenous levels of histone H2B when acetylated at Lys5 (Fig 1). Rabbit anti-Histone H2B monoclonal antibody has been precoated onto a microplate (Step 1). Cell lysate is pipetted into the wells (Step 2). Following extensive washing, biotinylated rabbit anti-acetyl-Histone H2B (K5) monoclonal antibody reagent is added (Step 3). Following a wash to remove any unbound antibody reagent, streptavidin-HRP is added to the well (Step 4). After washing away the unbound streptavidin- HRP, a substrate solution is added to the wells to develop color (Step 5). The magnitude of the absorbance for this developed color is proportional to the amount of acetyl-Histone H2B (K5).

Recommended Applications

ELISA

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Products Data

Fig1. Main steps of the Histone H2B Acetyl (K5) ELISA Kit.

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Components

- Dry Plate with stripwells (96)
- Biotinylated Anti-Acetyl Histone H2B (K5) Antibody Reagent
- Streptavidin-HRP Reagent
- Antigen/Antibody Diluent Buffer
- ELISA Washing Buffer
- TMB A Substrate Solution
- TMB B Substrate Solution
- Stop Solution

Storage Condition and Buffer

Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

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