AAK1 antibody RabMAb®

Cat.#: 6677-1
Abcam ID: ab134971

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

AAK1 is a member of the SNF1 subfamily of Ser/Thr protein kinases. It interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin (1).

Recommended Applications

WB, FC

Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
FC: 1:10 - 100

Species Reactivity *

Human

*Species cross-reactivity is based on WB analysis.

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Products Data

Western blot analysis on (A) HepG2 and (B) 293T cell lysates using anti-AAK1 RabMAb (cat. #6677-1).	Flow cytometric analysis of permeabilized 293T cells using anti-AAK1 RabMAb (red) (cat. # 6677-1) or a rabbit IgG (negative) (green).

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Specificity

A synthetic peptide corresponding to residues in human AAK1 was used as an immunogen.

Storage Condition and Buffer

Store at -20° C. Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

AAK1 antibody, AP2-associated protein kinase 1 antibody, Adaptor-associated kinase 1 antibody

Description References

1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1; 33: D54-8.

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