AAK1 antibody RabMAb®Cat.#: 6677-1
Abcam ID: ab134971
Rabbit Monoclonal Antibody
Swiss Prot: Q2M2I8
Mol Weight: 95kDa
AAK1 is a member of the SNF1 subfamily of Ser/Thr protein kinases. It interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin (1).
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
FC: 1:10 - 100
Species Reactivity *
*Species cross-reactivity is based on WB analysis.
|Western blot analysis on (A) HepG2 and (B) 293T cell lysates using anti-AAK1 RabMAb (cat. #6677-1).||Flow cytometric analysis of permeabilized 293T cells using anti-AAK1 RabMAb (red) (cat. # 6677-1) or a rabbit IgG (negative) (green).|
A synthetic peptide corresponding to residues in human AAK1 was used as an immunogen.
Storage Condition and Buffer
Store at -20° C. Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
AAK1 antibody, AP2-associated protein kinase 1 antibody, Adaptor-associated kinase 1 antibody
1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1; 33: D54-8.
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