JunB DyLight® 488 (JUNB) antibody RabMAb®

Cat.#: 4609-1
Abcam ID: ab133728

Rabbit Monoclonal Antibody

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Description

JunB is part of the activator protein-1 (AP-1) family of transcription factors that is involved in regulating gene activity following primary growth factor response. It binds to the AP-1 consensus-binding site sequence 5'-TGA[CG]TCA-3' (1, 2). Proteins of the AP-1 family contain a leucine zipper domain that allows homo- and heterodimer formation (1). Moreover, JunB is a growth-inhibiting protein that antagonizes the proliferative effects of c-Jun. It suppresses cell proliferation by direct transcriptional activation of the cell cycle suppressor p16INK4A and by negatively regulating cyclin D1. On the other hand, JunB has also a cell cycle-promoting role through activating the transcription of the cell cycle regulator cyclin A. However, AP-1 proteins not only act as oncogenes or tumor suppressors, but they are also involved in a broader spectrum of cellular events, such as apoptosis, differentiation, and inflammation (3).

Recommended Applications

ICC

Applications and Recommended Dilution Factors

ICC: 1:50

Species Reactivity

Human

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Products Data

Immunofluorescent analysis of HeLa cells using anti-JunB RabMAb (DyLight® 488 conjugate, cat.# 4609-1) (green) and dapi (blue).

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Specificity

A synthetic peptide corresponding to residues near the N-terminus in human JunB was used as an immunogen.

Storage Condition and Buffer

Store at 2-8° C. PBS (pH 7.2), 0.02% sodium azide, 0.05% BSA. Protect from light. Stable for 6 months from date of receipt.

Alternative Names

JUNB antibody, AP-1 antibody, Transcription factor jun-B antibody

Description References

1.Rusovici R and LaVoie HA. Biol Reprod. 2003 Jul;69(1):64-74. Epub 2003 Feb 19.
2. The UniProt Consortium Ongoing and future developments at the Universal Protein Resource Nucleic Acids Res. 39: D214-D219 (2011).
3. Pinent M et al. Endocrinology. 2011 Jul;152(7):2678-89. Epub 2011 May 3.

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