GSK3 beta Phospho (pS9) (GSK3B) antibody 
RabMAb®
Cat.#: 2435-1
Rabbit Monoclonal Antibody
Swiss Prot: P49841
Mol Weight: 47kDa
Price: $285*
Availability: Ship next business day
Description
Glycogen synthase kinase 3 beta (GSK3 beta) is a Serine/Threonine protein kinase involved in the insulin and wingless pathways (1-2). Originally identified as a regulator of glycogen synthase, it has been shown to regulate a diverse array of cellular functions. GSK3 beta activity is down regulated by phosphorylation on Serine 9 by PKB, or activated by phosphorylation on Tyrosine 216. Once activated, the protein can phosphorylate p53, c-jun, heat shock factor-1 and cyclin D1 (3-4). It is also known to phosphorylate Tau in Alzheimer's disease. Additionally, increased GSK3 beta protein levels are found in Alzheimer's disease brains.
Recommended Applications
WB, IHC, ICC
Applications and Recommended Dilution Factors
WB: 1:10,000 - 20,000
IHC: 1:50 - 100
ICC: 1:100 - 250
Species Reactivity *
Human
*Cross reactivity determined by western blot only.
Products Data
 |  |
| A.Western blot analysis on 293T cell lysates using anti-Phospho-GSK3 beta (pS9) RabMAb (cat. #2435-1), 1:20,000 dilution. Cells were either (A) untreated (B) treated with Calyculin A. | B. Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Phospho-specific anti-GSK3 beta (pS9) RabMAb (cat. # 2435-1). |
Specificity
A phospho-specific peptide corresponding to residues surrounding Serine 9 of human GSK3 beta was used as an immunogen. The antibody only detects GSK3 beta phosphorylated on Serine 9.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
GSK3B antibody, - antibody, Glycogen synthase kinase-3 beta antibody, antibody
Description References
1. Plyte SE, et al. Biochim Biophys Acta. 1114(2-3):147, 1992
2. Miller, J. R., et al. Genes Dev. 10: 2527-2539, 1996
3. Boyle WJ, et al. T. Cell. 64(3):573, 1991
4. Bellacosa, A., et al. P. N. Science 254: 244-247, 1992
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