LRRK2 Phospho (pS935) antibody RabMAb®Cat.#: 5099-1
Abcam ID: ab133450
Rabbit Monoclonal Antibody
Swiss Prot: Q5S007
Mol Weight: 286kDa
Price: $65 / $105
This antibody is available in larger quantities - Email request
This antibody was developed with the support of The Michael J. Fox Foundation (MJFF) and in partnership with Dr. Dario Alessi (MRC Protein Phosphorylation Unit, University of Dundee) to help accelerate LRRK2 research. Dr. Alessi has characterized several unique and high quality LRRK2 rabbit monoclonal antibodies, generated by Epitomics, to be made widely available for PD research community.
LRRK2 (Leucine-rich repeat kinase 2, dardarin) is a multi-domain protein belonging to the ROCO family of proteins that contains a kinase and GTPase domain among its many protein interaction domains (1). LRRK2 is mutated in a significant number of Parkinson's disease (PD) patients. Mutations in this gene account for 4% of PD, and are observed in 1% of sporadic PD patients (3). The most common mutation replaces glycine 2019 with a serine that results in increased LRRK2 kinase activity (4,5). This indicates that inhibitors of LRRK2 kinase activity might be of therapeutic benefit for the treatment of Parkinson’s disease and has stimulated much activity in this field of research.
Recent work has revealed that LRRK2 interacts with14-3-3 phospho-binding adaptor isoforms that is mediated by phosphorylation of Ser910 and Ser935 located prior to the leucine rich repeat domain mediates (6). Interestingly, 14-3-3 binding has been linked toParkinson’s disease as Ser910 as well as Ser935 and interaction with the 14-3-3 is inhibited by five of the six validated LRRK2 pathogenic mutations (R1441C, R1441G, R1441H, Y1699C and I2020T) (6,7).
The Dundee-MJFF LRRK2 PhosphoSer935 antibody will be of great utility in further understanding the link between 14-3-3 binding to LRRK2 and Parkinson’s disease as well as assessing the efficacy of LRRK2 inhibitors that are being developed (8). It should be noted the Dundee-MJFF antibody is highly selective and sensitive and can readily be used to monitor LRRK2 Ser935 phosphorylation in immunoblot analysis of 2-20 microgram amounts of whole cell extract. The Dundee-MJFF LRRK2 PhosphoSer935 recognizes both mouse and human endogenous LRRK2.
Applications and Recommended Dilution Factors
WB: 1:1000 - 5000
A synthetic phospho-peptide corresponding to residues surrounding Serine 935 of human LRRK2 was used as immunogen.
Storage Condition and Buffer
Store at -20° C. Antibody buffer, glycerol, and BSA. Stable for 12 months from date of receipt.
LRRK2 antibody, AURA17 antibody, DARDARIN antibody, PARK8 antibody, RIPK7 antibody, ROCO2 antibody, Leucine-rich repeat serine/threonine-protein kinase 2 antibody, Dardarin antibody
1. J Neurosci. 2010 Feb 3;30(5):1788-97.
2. Biochem J. 2010 Aug 27;430(3):393-404.
3. Proc Natl Acad Sci U S A. 102, 16842-16847
4. FEBS Lett. 579, 1417-1423
5. Biochem J. 430, 393-404
6. PLoS One. 6, e17153
7. Biochem J. 430, 405-413
8. Nat Chem Biol. 7, 203-205
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