p53 Acetyl (K382) (TP53) antibody 
RabMAb®
Cat.#: 2485-1
Rabbit Monoclonal Antibody
Swiss Prot: P04637
Mol Weight: 53kDa
Price: $285*
Availability: Ship next business day
Description
p53 acts as both a tumor-suppressor and transcription factor that, upon activation by DNA damage and other cellular stress signals, leads to the transcription of genes triggering cell-cycle arrest, apoptosis, and DNA repair (1,2). The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers (3). p53 is frequently mutated or inactivated in about 60% of cancers. p53 is acetylated at lysine 382 upon Ras expression, an event that is essential for its biological function (4). Acetylation at lysine 382 specifically determines the PAb421 epitope (5).
Recommended Applications
WB, ICC
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
ICC: 1:100
Species Reactivity *
Human
*Cross reactivity determined by western blot only.
Products Data
 |
| A. Western blot analysis on HepG2 cell lysates using anti-p53 Acetyl (K382) RabMAb (cat. #2485-1). Cells were either (A) untreated (B) treated with etoposide and TSA. |
Specificity
A synthetic acetylated peptide corresponding to residues surrounding K382 of human p53 was used as an immunogen. The antibody only detects p53 acetylated on Lysine 382.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
TP53 antibody, FLJ92943 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, Cellular tumor antigen p53 antibody, Antigen NY-CO-13 antibody, Phosphoprotein p53 antibody, Tumor suppressor p53 antibody
Description References
1. Levine, A.J. 88: 323
2. Lakin, N.D et al. Oncogene 18: 7644
3. Vogelstein, B. Nature 348: 681
4. Pearson M, et al. Nature 406, 207-210, 2000
5. Warnock LJ, et al. FEBS J. 272(7):1669-75, 2005
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