Cbl Phospho (pY674) (CBL) antibody 
RabMAb®
Cat.#: 2469-1
Rabbit Monoclonal Antibody
Swiss Prot: P22681
Mol Weight: 120kDa
Price: $285*
Availability: Ship next business day
Description
c-Cbl, product of c-cbl proto-oncogene, is a 120 kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4). It has been demonstrated that certain tyrosines are the major phosphorylation sites of c-Cbl in T cells in response to pervanadate treatment, as well as in response to TcR/CD3 ligation. Cbl also enhances migration of v-Abl-transformed NIH 3T3 fibroblasts. This effect of Cbl depends on its tyrosine phosphorylation, which is required for binding of PI-3' kinase to c-Cbl (5)
Recommended Applications
WB, ICC, FC
Applications and Recommended Dilution Factors
WB: 1:1000
ICC: 1:100 - 250
FC: 1:20
Species Reactivity *
Human
*Cross reactivity determined by western blot only.
Products Data
 |
| A. Western blot analysis on Jurkat cell lysates using anti-Phospho-Cbl (pY674) RabMAb (cat. #2469-1), 1:1000 dilution. Cells were either (A) untreated (B) treated with pervanadate. |
Specificity
A phospho specific peptide corresponding to residues surrounding tyrosine 674 of human Cbl were used as an immunogen. This antibody detects Cbl phosphorylated on tyrosine 674.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
CBL antibody, C-CBL antibody, CBL2 antibody, RNF55 antibody, E3 ubiquitin-protein ligase CBL antibody, Casitas B-lineage lymphoma proto-oncogene antibody, Proto-oncogene c-Cbl antibody, RING finger protein 55 antibody, Signal transduction protein CBL antibody
Description References
1. Langdon, W. Y., et al. Proc. Natl. Acad. Sci. USA 86:1168-72 (1989)
2. Blake, T. J., et al. EMBO J. 12: 2017-26 (1993)
3. Joazeiro, C. A., et al. Science 286: 309-12 (1999)
4. Feshchenko EA, et al. J Biol Chem, 272(14):8323-31, 1998
5. Teckchandani AM, et al. Exp Cell Res 307(1): 247-58,2005
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