Bad Phospho (pS112) (BAD) antibody 
RabMAb®
Cat.#: 5671-1
Rabbit Monoclonal Antibody
Swiss Prot: Q92934
Mol Weight: 23kDa
Price: $285*
Availability: Ship next business day
Description
Bad is a pro-apoptotic member of the Bcl-2 family. Bad will bind preferentially Bcl-xL over Bcl-2 through its partial (9-16 a.a) BH3 domain. Additionally, Bad can reverse the death repressor activity of Bcl-xL but not of Bcl-2. Upon dephosphorylation, activated Bad translocates to the mitochondria and displace Bcl-2 from tBid sequestration to initiate mitochondrial dysfunction. The switching on/off of its phosphorylation by growth/survival factors regulates Bad activity. EGF signals via p38 MAPK to increase phosphorylation of Bad at Serine 112 and thereby limit trophoblast apoptosis.
Recommended Applications
WB, IP
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IP: 1:10 - 100
Species Reactivity *
Human, Mouse, Rat
*Species cross-reactivity is based on WB analysis.
Products Data
 |
| Western blot analysis on HeLa cell lysates using anti-Phospho-Bad (pS112) RabMAb (cat. #5671-1). Cells were either (A) untreated or (B) treated with Calyculin A. |
Specificity
A phospho specific peptide corresponding to residues surrounding Serine 112 of human Bad was used as an immunogen. This antibody only detects Bad phosphorylated at Serine 112.
Storage Condition and Buffer
Store at -20° C. Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
BAD antibody, BBC2 antibody, BCL2L8 antibody, Bcl2 antagonist of cell death antibody, Bcl-XL/Bcl-2-associated death promoter antibody
Description References
1. Greider, C.W., and Blackburn, E.H. (1985). Cell 43, 405-413
2. Feng J, Funk WD, Wang SS, et al. Science 1995; 269:1236-41
3. Greider, C.W. (1996). Telomere length regulation. Annu. Rev. Biochem. 65, 337-365
4. Shay JW, Cacchetti SA, Eur J Cancer. 1997:33:787-791
5. Humphrey RG et al. Endocrinology. 2008 May;149(5):2131-7.
Similar Products
| Product Name | Catalog # |
|---|---|
| Bad (N-term) RabMAb® | 1541-1 |
| Bad (N-term) DyLight® 488 RabMAb® | 4509-1 |
| Phospho-Bad-S118 Antibody | T3690 |
| Phospho-Bad-S134 Antibody | T3691 |
| Phospho-Bad-S99 Antibody | T3693 |
Related Products
| Product Name | Catalog # |
|---|---|
| Goat Anti-Rabbit IgG (H+L) HRP | 3053-1 |
| Goat Anti-Rabbit IgG polymerized HRP | 3051-1 |
| Cell Lysis Buffer | 3500-1 |
| Goat Anti-Rabbit IgG HRP Antibody | 3056-1 |
| Goat Anti-Rabbit IgG DyLight® 405 | 3063-1 |
| Goat Anti-Rabbit IgG DyLight® 488 | 3064-1 |
| Goat Anti-Rabbit IgG DyLight® 550 | 3065-1 |
| Goat Anti-Rabbit IgG DyLight® 594 | 3066-1 |
| Goat Anti-Rabbit IgG DyLight® 633 | 3067-1 |
| Goat Anti-Rabbit IgG DyLight® 650 | 3068-1 |
