14-3-3 b/a (C-term) (YWHAB) antibody 
RabMAb®
Cat.#: 1062-1
Rabbit Monoclonal Antibody
Swiss Prot: P31946
Mol Weight: 30kDa
Price: $260
Availability: Ship next business day
Description
The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints (1). The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins (2,3). Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol (3).
Recommended Applications
WB, IHC, ICC, IP, FC
Applications and Recommended Dilution Factors
WB: 1:1,000
IHC: 1:500
ICC: 1:500
IP: 1:40
FC: 1:40
Species Reactivity *
Human, Mouse, Rat
*Cross reactivity determined by western blot only.
Products Data
 |  |
| A. Western blot analysis on Hela cell lysate using anti-14-3-3 b/a (C-term) RabMAb (cat. #1062-1), dilution 1:1,000. | B. Immunohistochemical analysis of paraffin-embedded breast tissue anti-14-3-3 b/a (C-term) RabMAb (cat. #1062-1). |
Specificity
A synthetic peptide corresponding to C-terminal residues of human 14-3-3 b/a was used as immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
YWHAB antibody, GW128 antibody, HS1 antibody, KCIP-1 antibody, YWHAA antibody, 14-3-3 protein beta/alpha, N-terminally processed antibody, Protein 1054 antibody, Protein kinase C inhibitor protein 1 antibody
Description References
1. Fu, H., et al. 14-3-3 proteins: structure, function, and regulation. Ann. Rev. Pharmacol. Toxicol. 40: 617
2. Muslin, A.J. et al. Interaction of 14-3-3 with Signaling Proteins Is Mediated by the Recognition of Phosphoserine. Cell 84: 889
3. Zha, J. et al. Serine Phosphorylation of Death Agonist BAD in Response to Survival Factor Results in Binding to 14-3-3 Not BCL-XL. Cell 87: 619
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