p53 Phospho (pT377) (TP53) antibody 
RabMAb®
Cat.#: 5150-1
Rabbit Monoclonal Antibody
Swiss Prot: P04637
Mol Weight: 53kDa
Price: $285*
Availability: Ship next business day
Description
p53 acts as both a tumor-suppressor and transcription factor that, upon activation by DNA damage and other cellular stress signals, leads to the transcription of genes triggering cell-cycle arrest, apoptosis, and DNA repair (1,2). The protein for the nuclear phosphoprotein p53 is the most commonly mutated protein yet identified in human cancers (3). p53 is frequently mutated or inactivated in about 60% of cancers (4,5). Phosphopeptide mapping, phosphoamino acid analysis and radiosequence analysis of p53 phosphorylated by PKC in vitro indicated that serine 370 and threonine 377 were the major targets for phosphorylation (6).
Recommended Applications
WB
Applications and Recommended Dilution Factors
WB: 1:10,000 - 50,000
Species Reactivity *
Human
*Species cross-reactivity is based on WB analysis.
Products Data
 |
| Western blot analysis on 293T cell lysates using anti-Phospho-p53 (pT377) RabMAb (cat. #5150-1). Cells were either (A) untreated (B) treated with Okadaic Acid and Calyculin A. |
Specificity
A phospho specific peptide corresponding to residues surrounding Threonine 377 of human p53 was used as an immunogen. This antibody only detects p53 phosphorylated at Threonine 377.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
TP53 antibody, FLJ92943 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, Cellular tumor antigen p53 antibody, Antigen NY-CO-13 antibody, Phosphoprotein p53 antibody, Tumor suppressor p53 antibody
Description References
1. Levine AJ. Cell 88(3):323-31, 1997
2. Lakin ND, et al. Oncogene 18(53):7644-55, 1999
3. Vogelstein, B. Nature 348(6303):681-2, 1990
4. Hollstein M, et al. Science 253(5015):49-53, 1991
5. Harris CC. Science 262(5142):1980-1, 1993
6. Milne DM, et al. Oncogene 13(1):205-11, 1996
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