LAT Phospho (pY200) antibody RabMAb®

Cat.#: 2274-1
Abcam ID: ab68139

Rabbit Monoclonal Antibody

---

Download datasheet [PDF]|Similar Products|Related Products

Bulk and Custom formulation available Email request

---

Description

LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 (on the short isoform of LAT) are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1. Tyr200 and Tyr220 on long isoform of LAT correspond to Tyr171 and Tyr191 on short isoform respectively (3).

Recommended Applications

WB, ICC

Applications and Recommended Dilution Factors

WB: 1:5000
ICC: 1:100 - 250

Species Reactivity *

Human

*Cross reactivity determined by western blot only.

---

Products Data

A. Western blot analysis on Jurkat cell lysates using anti-phosho LAT (pY200) RabMAb (cat. #2274-1), 1:10,000 dilution. Cells were either (A) untreated (B) treated with pervanadate.

---

Specificity

A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 200 of human LAT (long isoform) was used as immunogen. The antibody only detects LAT phosphorylated on Tyrosine 200 of long isoform and Tyrosine 171 of short isoform.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

LAT antibody, LAT1 antibody, pp36 antibody, Linker for activation of T-cells family member 1 antibody, p36-38 antibody

Description References

1. Weber, J.R., et al. J Exp Med. 187: 1157
2. Zhang, W., et al. Cell 92: 83
3. Paz, P.E., et al. Biochem J. 356(Pt 2): 461-71 (2001).

Product References

1. Huang SC, Tsai HF, Tzeng HT, et al, Lipid Raft Assembly and Lck Recruitment in TRAIL Costimulation Mediates NF-κB Activation and T Cell Proliferation ,J Immunol 186 (2011) 931-939

Similar Products

Product Name	Catalog #
LAT RabMAb®	1499-1
LAT Phospho (pY191) RabMAb®	1225-1

Related Products

Product Name	Catalog #
Goat Anti-Rabbit IgG (H+L) HRP	3053-1
Goat Anti-Rabbit IgG polymerized HRP	3051-1
Cell Lysis Buffer	3500-1
Goat Anti-Rabbit IgG HRP Antibody	3056-1