Src (SRC) antibody 
RabMAb®
Cat.#: 3795-1
Rabbit Monoclonal Antibody
Swiss Prot: P12931
Mol Weight: 60kDa
Price: $260*
Availability: Ship next business day
Description
Src is a protein tyrosine kinase known to regulate cellular adhesion. Composed of three major domains; SH2, SH3 and a kinase catalytic domain, Src can be switched from an inactive to an active state though control of its phosphorylation state or through protein-protein interaction. Phosphorylation of Y529 by Csk and Chk inactivates Src, while dephosphorylation of Y529 will modify its conformation to an open active state (1-3). Mutation of Y529 leads to Src overactivity. Several cancers including colon and breast cancer have been associated with an increase of Src activity (4).
Recommended Applications
WB, IHC, ICC, FC
Applications and Recommended Dilution Factors
WB: 1:10,000 - 50,000
IHC: 1:250 - 500
ICC: 1:100 - 250
FC: 1:10 - 100
Species Reactivity *
Human, Mouse, Rat
*Species cross-reactivity is based on WB analysis
Products Data
 |  |  |
| Western blot analysis on (A) Hela, (B) 293T, (C) SH-SY5Y, and (D) HUVEC cell lysates using anti-Src RabMAb (cat. #3795-1). | Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Src RabMAb (cat.# 3795-1). | Immunofluorescent staining of HeLa cells using anti-Src RabMAb (cat.# 3795-1). |
 | ||
| Immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma tissue using anti-Src RabMAb (cat.# 3795-1). |
Specificity
A synthetic peptide corresponding to residues in human Src was used as an immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
SRC antibody, ASV antibody, SRC1 antibody, c-SRC antibody, p60-Src antibody, Proto-oncogene tyrosine-protein kinase Src antibody, Proto-oncogene c-Src antibody, pp60c-src antibody
Description References
1. Cooper, J. A., and King, C. S. (1986) Mol. Cell. Biol. 6, 4467-4477
2. Courtneidge, S. A. (1985) EMBO J. 4, 1471-1477
3. Okada, M., Howell, B. W., Broome, M. A., and Cooper, J. A. (1993) J. Biol. Chem. 268, 18070-18075
4. Leiming Li, Masaya Okura, Akira Imamoto Molecular and Cellular Biology, Feb. 2002, p. 1203-1217
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