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Products
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Beta-Arrestin 1 Phospho (pS412) Antibody
Rabbit Monoclonal Antibody |
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Cat.# 1109-1
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Description
Beta-arrestin-1 is a member of the arrestin family that regulates beta-adrenergic receptor function. Beta-arrestins seem to mediate the desensitization and internalization of G protein-coupled receptors (1). -arrestin-1 is phosphorylated by Erk1/2 at Serine 412 of the C-terminus (2). Expanded roles for -arrestin-1 have been studied. Interaction with molecules such as clathrin, AP-2 and NSF directs the clathrin-mediated internalization of G-protein-coupled receptors. Interaction with molecules such as Src, Raf, Erk, ASK1 and JNK3 appears to regulate several pathways that result in the activation of MAP kinases (3,4). |
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Recommended Applications
WB, IHC, ICC, IP |
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| Applications and Recommended Dilution Factors |
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| WB | IHC | ICC | FC | IP |
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| 1:20,000 |
1:500 |
1:100-1:250 |
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1:100 |
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| Species Reactivity* |
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| Human |
Mouse |
Rat |
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| Positive |
Positive |
Positive |
*Cross reactivity determined by western blot only. |
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| Product Data |
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| A. Western blot analysis on 293T cell lysate using anti-Phospho-ß-Arrestin-1 (pS412) RabMAb (cat. #1109-1); dilution 1:20,000. |
B. Immunohistochemical analysis of paraffin-embedded human lymph node using anti-Phospho-ß-Arrestin-1 (pS412) RabMAb (cat. #1109-1). |
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Specificity
A synthetic phospho-peptide corresponding to residues surrounding Ser412 of human Beta Arrestin-1 was used as immunogen. The antibody only detects Beta Arrestin-1 phosphorylated on Serine 412. |
Storage Buffer & Conditions
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Alternative Names
ARRB1, ARR1, Beta-arrestin-1, Arrestin beta-1 |
Description References
1. Lefkowitz, R.J. G protein-coupled receptors. III. New roles for receptor kinases and beta-arrestins in receptor signaling and desensitization. J. Biol. Chem. 273: 18677
2. Lin, F.T., et al. Feedback regulation of beta-arrestin1 function by extracellular signal-regulated kinases. J. Biol. Chem. 274: 15971
3. Lin, F.T., et al. beta-arrestins regulate mitogenic signaling and clathrin-mediated endocytosis of the insulin-like growth factor I receptor. J Biol Chem. 273: 31640
4. Miller, W.E., and R.J. Lefkowitz. Expanding roles for beta-arrestins as scaffolds and adapters in GPCR signaling and trafficking. Curr Opin Cell Biol. 13: 139
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Product References
1. Yuan J, Liu X, Wu AW, et al, Breaking human cytomegalovirus major immediate-early gene silence by vasoactive intestinal peptide stimulation of the protein kinase A-CREB-TORC2 signaling cascade in human pluripotent embryonal NTera2 cells ,J. Virol. 83 (2009) 6391 - 6403
[Application: WB ]
2. Mandell JW, Glass G, Gianchandani EP, et al, Dephosphorylation of β-Arrestin 1 in Glioblastomas ,J Neuropathol Exp Neurol. 68 (2009) 535-541
[Application: WB, IHC ]
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