Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; α and β, or α and γ subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). PKA specifically phosphorylates Ser408, Ser436, and Ser481 located in the neck domain of alpha Adducin. Phosphorylation of Adducin alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of Adducin alpha (5).
Recommended Applications
WB, IHC, ICC, IP
Applications and Recommended Dilution Factors
WB
IHC
ICC
FC
IP
1:20,000
1:250 - 500
1:100 -250
1:50
Species Reactivity*
Human
Mouse
Rat
Positive
Positive
Positive
*Cross reactivity determined by western blot only.
Product Data
A. Western blot analysis on (1) Hela cell lysate and (2) HeLa lysate + TPA using anti-Phospho-Adducin (pS481) RabMAb (catalog #1762-1), dilution 1:20,000.
B. Immunohistochemical analysis of paraffin-embedded human uterus using anti-Adducin alpha Rab MAb. (cat. # 1762-1).
Specificity
A synthetic phospho-peptide corresponding to residues surrounding Ser481 of human alpha Adducin was used as an immunogen. The antibody only detects alpha Adducin phosphorylated on Serine 481.
Storage Buffer & Conditions
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
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