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    RSK1 Phospho (pT359/pS363) Antibody
    Rabbit Monoclonal Antibody
    Cat.#  2006-1

    Clone ID: E238
    Swiss Prot: Q15418  
    Mol Weight: 90 kDa Price: $285
    Size: 100ul

    Print Page Print Page Download Datasheet Datasheet pdf  


    Description

    Rsk-1 (p90-Rsk-1) is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk-1, Rsk-2 and Rsk-3. These are broadly expressed serine/threonine protein kinases (1) activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2 (2). p90-Rsk-1 is activated by MAPK in vitro and in vivo via phosphorylation (3). Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB (2,4).



    Recommended Applications

    WB, IHC, ICC, IP

    Applications and Recommended Dilution Factors
     
    WBIHCICCFCIP

    1:20,000 1:100 1:50 1:40

    Species Reactivity*
     
    Human Mouse Rat

    Positive Positive Negative *Cross reactivity determined by western blot only.

    Product Data
    Immunohistochemical staining of untreated paraffin-embedded human cervical carcinoma using anti-Phospho-Rsk1 (pT359/pS363) RabMAb (cat. #2006-1). Immunohistochemical staining of Phosphatase-treated paraffin-embedded human cervical carcinoma using anti-Phospho-Rsk1 (pT359/pS363) RabMAb (cat. #2006-1).
    Western blot analysis on A431 cell lysate using anti- Phospho-Rsk1 (pT359/pS363) RabMAb (cat. #2006-1); dilution 1:20.000. Cell lysates were (1) untreated, or (2) treated with EGF.

    Specificity

    A synthetic phospho-peptide corresponding to residues surrounding Thr359 and Ser363 of human RSK1 was used as immunogen. The antibody only detects RSK1 phosphorylated on Threonine 359 and Serine 363.


    Storage Buffer & Conditions

    50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.


    Alternative Names

    RPS6KA1, RSK1, Ribosomal protein S6 kinase alpha-1, MAP kinase-activated protein kinase 1a


    Description References

    1. Dalby, K.N. et al. Identification of Regulatory Phosphorylation Sites in Mitogen-activated Protein Kinase (MAPK)-activated Protein Kinase-1a/p90rsk That Are Inducible by MAPK. J. Biol. Chem. 273, 1496-1505 (1998)
    2. Frodin, M. and Gammeltoft, S. Role and regulation of 90 kDa ribosomal S6 kinase (RSK) in signal transduction. Mol. Cell. Endocrinol. 151, 65
    3. Lazar, D. F. et al. Mitogen-activated Protein Kinase Kinase Inhibition Does Not Block the Stimulation of Glucose Utilization by Insulin. J. Biol. Chem. 270, 20801
    4. Chen, R. et al. Phosphorylation of the c-Fos Transrepression Domain by Mitogen-Activated Protein Kinase and 90-kDa Ribosomal S6 Kinase. Proc. Natl. Acad. Sci. USA 90, 10952



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