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    GRB2 (N-term) Antibody
    Rabbit Monoclonal Antibody
    Cat.#  1584-1

    Clone ID: Y301
    Swiss Prot: P62993  
    Mol Weight: 25 kDa Price: $250
    Size: 100ul

    Print Page Print Page Download Datasheet Datasheet pdf  


    Description

    The growth factor bound protein 2 (GRB2) is a 25 kDa cytosolic protein that contains one src homology (SH2) domain and two SH3 domains (1). GRB2 associates with Tyrosine phosphorylated EGFRs and PDGFRs via its SH2 domain. GRB2 also plays a crucial role in two separate insulin pathways leading from the insulin receptor to the Ras protein thus affecting mitogenic signaling (2). Additionally GBR2, a bifunctional adaptor protein, recruits Sos for Ras activation and subsequent signal transmission (3).



    Recommended Applications

    WB, IHC, ICC, FC

    Applications and Recommended Dilution Factors
     
    WBIHCICCFCIP

    1:500 1:100-250 1:100-250 1:40

    Species Reactivity*
     
    Human Mouse Rat

    Positive Positive Positive *Cross reactivity determined by western blot only.

    Product Data
    A. Western blot analysis of anti-GRB2 (N-term) RabMAb (catalog #1584-1), dilution 1:500 A-NIH 3T3 B-PC12 C-Hela B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-GRB2 (N-term) RabMAb (catalog #1584-1).
    C. Immunofluorescent staining of PC-12 cells using anti-GRB-2 (N-term) RabMAb (catalog #1584-1).

    Specificity

    A synthetic peptide corresponding to residues in the N-terminus of human GRB2 was used as an immunogen. This antibody can also detect the splice isoform GRB3-3 of GRB2.


    Storage Buffer & Conditions

    50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.


    Alternative Names

    GRB2, ASH, Growth factor receptor-bound protein 2, Adapter protein GRB2;SH2/SH3 adapter GRB2;Protein Ash


    Description References

    1. Matuoka, K.; Shibata, M.; Yamakawa, A.; Takenawa, T.: Proc. Nat. Acad. Sci. 89: 9015-9019, 1992
    2. Lowenstein, E. J.; Daly, R. J.; Batzer, A. G.; Li, W.; Margolis, B.; Lammers, R.; Ullrich, A.; Skolnik, E. Y.; Bar-Sagi, D.; Schlessinger, J.: Cell 70: 431-442, 1992.
    3. Nioche P.; et al, JMB, Volume 315, Number 5, February ( 2002), 1167-1177(11)



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