Oncogene-encoded proteins c-Myc, N-Myc, and L-Myc function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors, the N-Myc gene in neuroblastomas (1), and the L-Myc gene in human small cell lung carcinomas (2). c-Myc protein is a transcription factor localized to the nucleus of the cell. It seems to be involved in activating the transcription of growth-related genes. c-Myc binds to DNA during transcription as a heterodimeric complex with Max. c-Myc is phosphorylated in vitro by p44/42 MAP kinase at Ser62 (3) and in vivo at both Thr58 and Ser62. Mutation of Thr58 and Ser62 to Ala inhibits the ability of c-Myc to activate transcription (3).
Recommended Applications
WB, ICC, IP
Applications and Recommended Dilution Factors
WB
IHC
ICC
FC
IP
1:1,000
1:50-100
1:100
Species Reactivity*
Human
Mouse
Rat
Positive
Positive
Negative
*Cross reactivity determined by western blot only.
Product Data
A. Western blot analysis on A431 cell lysate using anti-Phospho-c-Myc (pT58/pS62) RabMAb (catalog #1203-1), dilution 1:1,000. Cell lysates were either (1) untreated or (2) treated with TPA.
B. Immunofluorescent staining of A4321 cells using anti-phospho-c-Myc (T58/S62) RabMAbb (catalog #1203-1).
Specificity
A synthetic phospho-peptide corresponding to residues surrounding Thr58 and Ser62 of human C-Myc was used as immunogen. The antibody only detects c-Myc phosphorylated on Threonine 58 and Serine 62. Predicted to cross-react with most species, based on sequence homology.
Storage Buffer & Conditions
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Alternative Names
c-Myc, Transcription factor p64
Description References
1. Nisen, P.D. et al. Enhanced expression of the N-myc gene in Wilms 2. Nau, M.N. et al. L-myc, a new myc-related gene amplified and expressed in human small cell lung cancer. Nature 318: 69 3. Gupta, S. et al. Transactivation of Gene Expression by Myc is Inhibited by Mutation at the Phosphorylation Sites Thr-58 and Ser-62. Proc. Natl. Acad. Sci. USA 90: 3216
Product References
1. Huynh H, Ngo VC, Fargnoli J, et al, Brivanib Alaninate, a Dual Inhibitor of Vascular Endothelial Growth FactorReceptor and Fibroblast Growth Factor Receptor Tyrosine Kinases, Induces Growth Inhibition in Mouse ,Clin Cancer Res. 14 (2008) 6146-6153 [Application: WB]
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