A synthetic phospho-peptide corresponding to residues surrounding Thr58 and Ser62 of human C-Myc was used as immunogen. The antibody only detects c-Myc phosphorylated on Threonine 58 and Serine 62. Predicted to cross-react with most species, based on sequence homology.

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

MYC, Myc proto-oncogene protein, c-Myc;Transcription factor p64

1. Nisen, P.D. et al. Enhanced expression of the N-myc gene in Wilms
2. Nau, M.N. et al. L-myc, a new myc-related gene amplified and expressed in human small cell lung cancer. Nature 318: 69
3. Gupta, S. et al. Transactivation of Gene Expression by Myc is Inhibited by Mutation at the Phosphorylation Sites Thr-58 and Ser-62. Proc. Natl. Acad. Sci. USA 90: 3216

1. Stemke-Hale K, Gonzalez-Angulo AM, Lluch A, et al, An Integrative Genomic and Proteomic Analysis of PIK3CA, PTEN, and AKT Mutations in Breast Cancer ,Cancer Res 68 (2008) 6084-6091
[Application: RPPA ]
2. Huynh H, Ngo VC, Fargnoli J, et al, Brivanib Alaninate, a Dual Inhibitor of Vascular Endothelial Growth FactorReceptor and Fibroblast Growth Factor Receptor Tyrosine Kinases, Induces Growth Inhibition in Mouse ,Clin Cancer Res. 14 (2008) 6146-6153
[Application: WB ]