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    CD22 Phospho (pY822)
    Rabbit Monoclonal Antibody
    Cat.#  1610-1

    Clone ID: Y506
    Swiss Prot: P20273  
    Mol Weight: 150 kDa Price: $285
    Size: 100ul

    Print Page Print Page Download Datasheet Datasheet pdf  


    Description

    CD22, a negative regulator of B cell signaling, belongs to the sialoadhesin family of receptors that preferentially binds to α2-6-linked sialic acid on glycoproteins (1-2). CD22 is known to interact with the B cell antigen receptor (BCR) (3) and the tyrosine phosphatase SHP1. Tyrosine phosphorylation of CD22 leads to the recruitment of multiple intracellular Src homology 2 (SH2) domain-containing effector molecules, including phospholipase C and Syk, which are important for triggering Ca2+ influx, as well as PI 3-kinase and Grb2 (4-5). Phosphorylation of Tyrosine 822 is involved in the binding of Syk.



    Recommended Applications

    WB, FC

    Applications and Recommended Dilution Factors
     
    WBIHCICCFCIP

    1:1,000 1:10

    Species Reactivity*
     
    Human Mouse Rat

    Positive Negative Negative *Cross reactivity determined by western blot only.

    Product Data
    A. Western blot analysis on Raji cell lysate using anti-Phospho-CD22 (pY822) RabMAb (cat. #1610-1), 1:1,000 dilution. Cells were either (A) untreated (B) treated with Pervanadate B. Flow cytometric analysis of permeabilized K562 cells, untreated (green) or Pervanadate-treated (red) using anti-CD22 (pY822) RabMAb (cat. # 1610-1).

    Specificity

    A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 822 of human CD22 was used as immunogen. The antibody will detect CD22 phosphorylation on Tyrosine 822. This antibody is predicted to detect splice isoform 2 based on sequence homology.


    Storage Buffer & Conditions

    50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.


    Alternative Names

    Sialic acid-binding Ig-like lectin 2, Siglec-2, Leu-14, B-lymphocyte cell adhesion molecule, BL-CAM


    Description References

    1. Wilson, G. L.; Najfeld, V.; Kozlow, E.; Menniger, J.; Ward, D.; Kehrl, J. H.: J. Immun. 150: 5013-5024, 1993.
    2. Nitschke, L., H. Floyd, P. R. Crocker. 2001. Scand. J. Immunol. 53:227
    3. Tedder, T. F., J. Tuscano, S. Sato, J. H. Kehrl. 1997. Annu. Rev. Immunol. 15:481
    4. Yohannan, J., J. Wienands, K. M. Coggeshall, L. B. Justement. 1999. J. Biol. Chem. 274:18769
    5. Otipoby, K. L., K. E. Draves, E. A. Clark. 2001. J. Biol. Chem. 276:44315.



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