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Products
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Cbl Phospho (pY774) Antibody
Rabbit Monoclonal Antibody |
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Cat.# 1160-1
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Description
c-Cbl, product of c-cbl proto-oncogene, is a 120 kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4). |
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Recommended Applications
WB, IHC, ICC, FC, IP |
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| Applications and Recommended Dilution Factors |
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| WB | IHC | ICC | FC | IP |
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| 1:1,000 |
1:25 |
1:25 |
1:20 |
1:20 |
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| Species Reactivity* |
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| Human |
Mouse |
Rat |
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| Positive |
Negative |
Negative |
*Cross reactivity determined by western blot only. |
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| Product Data |
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| A. Western blot analysis on (1) Jurkat and (2) Jurkat + Pervanadate cell lysates using anti-Cbl (pY774) RabMAb (catalog #1160-1), 1:1,000 dilution. |
B. Immunohistochemical analysis of human cervical carcinoma using anti-Cbl (pY774) RabMAb (catalog #1160-1). |
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| C. Flow cytometric analysis of permeabilized Jurkat cells, untreated (green) or Pervanadate-treated (red), using anti-Cbl RabMAb (catalog #1160-1). |
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Specificity
A synthetic phospho-peptide corresponding to residues surrounding Tyr774 of human Cbl was used as immunogen. The antibody detects Cbl phosphorylated on Tyrosine 774. |
Storage Buffer & Conditions
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Alternative Names
CBL, CBL2, RNF55, E3 ubiquitin-protein ligase CBL, Signal transduction protein CBL;Proto-oncogene c-CBL;Casitas B-lineage lymphoma proto-oncogene;RING finger protein 55 |
Description References
1. Langdon, W. Y., et al. v-cbl, an oncogene from a dual-recombinant murine retrovirus that induces early B-lineage lymphomas. Proc. Natl. Acad. Sci. USA 86: 1168
2. Blake, T. J., et al. The truncation that generated the v-cbl oncogene reveals an ability for nuclear transport, DNA binding and acute transformation. EMBO J. 12: 2017
3. Joazeiro, C. A., et al. The tyrosine kinase negative regulator c-Cbl as a RING-type, E2-dependent ubiquitin-protein ligase. Science 286: 309
4. Galisteo, M. L., et al. Tyrosine phosphorylation of the c-cbl proto-oncogene protein product and association with epidermal growth factor (EGF) receptor upon EGF stimulation. J. Biol. Chem. 270: 20242
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