Transcription factor PU.1 is a member of the Ets family of transcription factors and is required for the development of multiple hematopoietic lineages (3). It plays a pivotal role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development. Transcription factor PU.1 and AML1, a DNA-binding subunit of the CBF transcription factor family, act as tumor suppressors in leukemia. The function of PU.1 is down-regulated by AML1-ETO in myeloid leukemia, whereas overexpression of PU.1 restores normal differentiation (1-2). The lack of transcription factor PU.1 protein expression in classical Hodgkin’s disease (cHD), a lymphoproliferative disease of predominantly B-cell origin, likely contributes to the lack of immunoglobulin expression and incomplete B-cell phenotype characteristic of the Reed-Sternberg cells in cHD (4).
Recommended Applications
WB, IHC, FC
Applications and Recommended Dilution Factors
WB
IHC
ICC
FC
IP
1:5,000 - 20,000
1:100 - 250
1:70
Species Reactivity*
Human
Mouse
Rat
Positive
Negative
Negative
*Cross reactivity determined by western blot only.
Product Data
A. Western blot analysis on (A) Daudi and (B) U937 cell lysates using anti-Transcription factor PU.1 RabMAb (cat. # 2480-1), dilution 1:10,000.
B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-Transcription factor PU.1 RabMAb (cat. #2480-1).
Specificity
A synthetic peptide corresponding to residues near the N terminus of Transcription factor PU.1 was used as an immunogen.
Storage Buffer & Conditions
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Alternative Names
SPI1, Transcription factor PU.1, 31 kDa-transforming protein
Description References
1. Huang G, et al. Nat Genet. 40(1):51-60, 2008 2. Vangala R K, et al. Blood 101(1):270-277, 2003 3. Kim H, et al. Blood 104(13):3894-3900, 2004 4. Torlakovic E, et al. American Journal of Pathology
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