Anti-Polyethylene Glycol Antibodies

Anti-PEG Portal

Anti-Polyethylene Glycol (PEG)

Anti-PEG Rabbit Monoclonal Antibodies and ELISA Kit

Polyethylene glycol (PEG) is a family of long chain polymers attached to a glycerine backbone. It is a nonionic, nontoxic, biocompatible, strongly hydrophilic polymer, which has a large exclusion volume in aqueous solution (1). The covalent attachment of PEG (PEGylation) is commonly used to modify a variety of proteins and drugs (2-4).

Benefits of PEGylated molecules include:

increased bioavailability
increased blood circulation of the drug
optimized pharmacokinetics
decreased immunogenicity
decreased frequency of administration.

Due to the nonionic and biocompatible nature of PEG it is not very immunogenic and thus very difficult to raise antibodies against. After extensive development Epitomics was able to successfully generate a high quality PEG Rabbit Monoclonal Antibody. This PEG Rabbit Monoclonal can reliably be used for the quality control of PEGylated molecules as well as monitoring the pharmacokinetics of PEGylated molecules via ELISA, WB and Immunohistochemistry.

Anti-PEG Products

Anti-PEG Rabbit Monoclonal Antibodies

The modification of a biopharmaceutical with PEG increases its hydrodynamic radius, and reduces immunogenicity and proteolytic cleavage. Other benefits include decelerated renal excretion, improved stability towards proteolysis and increased solubility of the biopharmaceutical in aqueous solutions.
An Anti-PEG antibody can be used to monitor a drug's pharmacokinetics, including distribution, metabolism and excretion. Also, it can be used for the quality control of pegylated molecules in ELISA, WB and IHC.

Rabbit vs Mouse

Pharmacokinetics

IHC

PEG ELISA Kit - New complete competition based ELISA Kit

The PEG ELISA kit operates on the basis of competition between the enzyme (HRP) conjugated PEG and PEG labeled molecules for a limited number of binding sites on the surface of 96-wells coated with Anti-PEG antibody. The extent of color development resulted from interaction between HRP and substrate TMB is inversely proportional to the amount of PEGylated molecules in the sample (Fig 1).

Fig 1. Competition between PEG labeled HRP (H) and PEG labeled sample (S) in PEG ELISA assay.

Anti-PEG ELISA Kit (6100-1)

Anti-PEG Ordering Information

Product Name	Catalog #	Clone ID	Applications
Polyethylene Glycol (PEG) RabMAb®	2061-1	PEG-B-47	WB,IHC	$295
Polyethylene Glycol (PEG) Backbone RabMAb®	3104-1	PEG-2-128	ELISA,WB	$305
Polyethylene Glycol (PEG) ELISA Kit RabMAb®	6100-1	N/A	ELISA	$545
Polyethylene Glycol (PEG)-Biotin RabMAb®	2137-1	PEG-B-47b	WB,IHC,ELISA	$337

* Pricing note: Academic Price / For-Profit Price

Product References

1. Peng S, Adelman RA, Rizzolo LJ, Minimal Effects of VEGF and Anti-VEGF Drugs on the Permeability or Selectivity of RPE Tight Junctions, Invest Ophthalmol Vis Sci 51 (2010) 3216-3225