EpiMAX Affinity Purification Kit

A Simple Purification System with Maximum Yield

  • All-In-One Antibody Purification Kit
  • Quick and Simple Peptide/Protein Immobilization
  • Maximum Antibody Yield in Less Time
  • Verstile Gel Matrix with High Binding Capacity and Stability
  • Optimized for both manual gravity and automated pump

EpiMAX™ Affinity Purification Kit

EpiMAX™ Affinity Purification kit is a complete antibody purification solution for quick and easy purification of ultra pure antibodies. The EpiMAX™ Affinity Purification kit is compatible with manual or automated procedures and small or large scale antibody purifications.

Fig 1.Simple Reaction Scheme for coupling of peptide/protein to ASA gel matrix

EpiMAX™ Affinity Purification kit is based on Epitomics ASA (Activated Serial-A) Matrix Gel Technology which provides multiple benefits –

  • Easy column preparation with highly stable covalent immobilization of any SH- or NH3- containing target peptide/protein (Figure 1)
  • Immobilize both peptide and protein of any size and at various buffer and temperature conditions (Table 1).
  • Superior peptide coupling efficiency and binding capacity compared to traditional peptide based antibody purification (Figure 2)

EpiMAX™ Affinity Purification kit requires less purification time and provides higher yield of ultra pure (>95% purity) antibody compared to traditional peptide purification methods (Figures 3-5).

For Automated purification, EpiMAX™ Affinity Purification kit includes adjustable column adaptor for easy conversion between gravity and pump based purification

Fig 2. A comparsion of minimum and maximum values of peptide binding coupling efficiency (CE%) and antibody binding capacity (mg/ml of gel) between EpiMAX™ and traditional peptide based purification kit.

Fig 3. A comparsion of total purification steps and time between EpiMAX™ and traditional peptide based purification kit

Fig 4. A comparsion of minimum and maximumtarget antibody recovery (%) between EpiMAX™ and traditional peptide based purification kit
 

Fig 5. Western blot analysis on Jurkat cell lysate using Cyclin B1 RabMAb A) Crude antisera, B) flow-thru, C) EpiMAX™ Purified. Target band at 58 kDa.

  
Condition
Compatible Range
Temperature
4°C to 50°C
pH
6 to 10
Solvent Type
Organic and Inorganic

Product Listing

Product Name

Type

Cat#

Price

EpiMAX™ Affinity Purification Kit

Kit (purification up to 5 different antibodies)

6000-1

$395

EpiMAX™ Affinity Purification Kit Manual

Requirements :
• Peptide for conjugation to the column must have SH- or NH3- terminal group.
• Sample requirement is as follows: ELISA based sample reading at 1:16K Dilution factor
   should be equal or greater than 0.50 (OD 280 reading).
List of Components :
•Activated Coupling Gel*
•Antigen Coupling Buffer
•Reductant Sodium
•Reductant Solution
•Peptide Dissolving Buffer
•Antisera Sample Buffer
•Antibody Elution Buffer
•Antibody Neutralization Buffer
•Chromatography Columns (5)
•Column Adaptor for Pump Usage (1)

*Bulk Size Available - contact info@epitomics.com or call 877-772-2622

EpiMAX™ Automated Pump

For automated pump purification, EpiMAX™ Affinity Purification kit is optimized to be used with Epitomics EpiMAX™ automated pump (Cat# APS-D100NA, APS-D100EA ).


  EpiMAX™ Automated Pump User Manual


Epitomics EpiMAX™ Automated Pump - APS-D100NA, APS-D100EA

Kit Product Listing

Product Name
Type
Cat#
Price
Buy Now
EpiMAX™ Automated Pump - North America Version
Syringe Pump (120 volts)
$895
EpiMAX™ Automated Pump - Asia/Europe Version
Syringe Pump (220 volts)
Please Inquire


Pump Specifications

Syringe Adaptability
Standard 20, 30, 50 ml Syringes
Volume Limit
0-9999 ml
Accuracy
≤±2%

Syringe Volume 20ml 30ml 50ml
Flow Rate 0.1-399.9ml/h 0.1-600.0ml/h 0.1-1200.0ml/h
Rapid Rate 399ml/h 600.0ml/h 1200.0ml/h

Dimensions
307mm (L) x 135mm (W) x 128 mm (H)
Net Weight
2.1 kg
Operation Conditions
5 - 40°C, RH: 20 - 40 %
Storage Conditions
-40 - 55°C, RH: 95%

View the complete list of Epitomics RabMAbs.

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