Epitomics Tandem™ IHC Staining and Detection Kits
• Dual color IHC detection
• Pair Epitomics RabMAbs with any mouse mAb
• Optimized for use with Paraffin embedded tissues
• Strong specific staining with clean background
• Complete ready to use kits including detection reagents
Click Here for Price and More Info  Tandem IHC Kit User Manual
Looking for Single Staining Kits - Click Here for Ace™ Single Staining Kits
DualColor IHC Detection Images - Formalin fixed, paraffin-embedded tissue
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Bcl-2 RAbMAb- Ki-67 mouse mAb
Lung Cancer |
Keratin-8 RabMAb - Ki-67 mouse mAb
Urinary Bladder Cancer |
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Bcl-x RabMAb - p53 mouse mAb
Stomach Cancer |
Her-2 RabMAb - ER-alpha mouse mAb
Breast Cancer |
Click Here for More DoubleColor IHC Images
Intro
Double staining is a technique used to evaluate the expression of two distinct antigens in the same tissue sample. The advent of Rabbit Monoclonal antibodies (RabMAbs) has opened up the possibility of performing reliable and clean dual IHC staining of formalin fixed, paraffin-embedded tissue samples.
Until now, the majority of staining protocols involved biotinylated primary antibodies or using a combination of monoclonal and polyclonal antibodies. These procedures generally provide inconsistent staining with high background. RabMAbs can be easily paired with mouse monoclonal antibodies in a straightforward procedure that results in beautiful, clean dual labeled samples.
Tandem IHC Kits are offered with or withour the primary rabbit monoclonal; Staining Kits include a rabbit monolconal primary antibody which have been specifically tested for use in dual color IHC detection. The Detection Kits contain the detection reagents only enabling researchers to pair any suitable Epitomics rabbit monoclonal antibody with any mouse monoclonal of their choice.
Examples of dual color staining using Epitomics Tandem IHC kits below.
Each Tandem IHC Kits is supplied with sufficient reagents to perfrom up to 50 stainings.
Product Listing
| Tandem™ IHC Detection Kits (w/o primary RabMAb) |
Tandem Detection Kit I |
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$295 |
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List of Components*
•Antigen Retrieval solution (1X)
•Peroxidase Block solution
•Blocking solution
•Primary antibody diluent
•Negative control rabbit IgG (1X)
•Secondary anti-Rabbit (HRP)
•Secondary anti-Mouse (AP)
•DAB A
•DAB B
•Fast Red tablets
•Fast Red solution
*Tandem IHC Detection kits are supplied w/o the Rabbit monoclonal primary Ab.
Tandem IHC Kit User Manual
| DualColor IHC Detection Images - Formalin fixed, paraffin-embedded tissue |
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AIF RabMAb - Ki-67 mouse mAb
Cervical Carcinoma |
AIF RabMAb - p53 mouse mAb
Prostate Cancer |
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BAX RabMAb - p53 mouse mAb
Prostate Cancer |
BID RabMAb - ER-alpha mouse mAb
Breast Carcinoma |
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Bcl-2 RabMAb - p53 mouse mAb
Lymphoma |
BID RabMAb - p53 mouse mAb
Breast Carcinoma |
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BIM RabMAb - p53 mouse mAb
Ovary Cancer |
Keratin-18 RabMAb- Ki-67 mouse mAb
Colon Cancer |
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Keratin-8 RabMAb - p53 mouse mAb
Stomach Cancer |
Keratin-18 RabMAb - p53 Mouse mAb
Breast Cancer |
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Her-2 RabMAb - p53 mouse mAb
Breast Cancer |
p53 RabMAb - Vimentin mouse mAb
Breast Cancer |
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View the complete list of Epitomics RabMAbs.
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