Rabbit Monoclonal Antibody | Product Data Sheet
|Quantity: 100ul||Lot #: Please refer to vial|
|Clone ID: EPR5804||Molecular Wt.: 116 kDa|
|Species Cross-reactivity* +Human+Mouse+Rat||UniProt ID: P55884|
Background: eIF-3B is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (1).
Specificity: A synthetic peptide corresponding to residues in human eIF-3B was used as an immunogen.
Storage Condition and Buffer: Store at -20° C. Buffer: PBS (pH 7.2), 50% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
WB: 1:10,000 - 50,000
ICC: 1:100 - 250
IP: 1:10 - 100
FC: 1:10 - 100
1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8.
*Species cross-reactivity is based on WB analysis.
Fig 1. Western blot analysis on (A) A431, (B) 293T, (C) Jurkat, (D) HeLa, and (E) Caco-2 cell lysates using anti-eIF-3B RabMAb (cat. #5423-1).
Fig 2. Flow cytometric analysis of permeabilized HeLa cells using anti-eIF-3B RabMAb (red) (catalog # 5423-1) or a rabbit IgG (negative) (green).