Background: Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to the calbindin family. It is mainly found in neuronal tissue, highly expressed in auditory neurons. It has been implicated as a calcium buffer, calcium sensor, and regulator of apoptosis (1). Calretinin is considered a specific marker of mesothelial cells; it is the only marker that has can distinguish between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in a panel of markers for distinguishing mesothelioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).

Specificity: A synthetic peptide corresponding to residues in human Calretinin was used as an immunogen.

Storage Condition and Buffer: Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Recommended Dilutions:
WB: 1:1,000
IHC: 1:100 - 250
ICC: 1:100 - 250
FC: 1:100

Background References:
1. Plattelli A et al. J Endocl 29(6):394-396, 2003. 2. Ordonez NG et al. Hum Pathol. 35(6):697-710, 2004. 3. Ordonez NG et al. Hum Pathol. 30(3):313-23. 1999.

*For Mouse, this antibody is recommended for WB only
Rat cross reactivity tested by western blot only