Rabbit Monoclonal Antibody | Product Data Sheet
|Quantity: 100ul||Lot #: Please refer to vial|
|Clone ID: EP1798||Molecular Wt.: 29 kDa|
|Species Cross-reactivity* +Human+Mouse+Rat||UniProt ID: P22676|
Background: Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to the calbindin family. It is mainly found in neuronal tissue, highly expressed in auditory neurons. It has been implicated as a calcium buffer, calcium sensor, and regulator of apoptosis (1). Calretinin is considered a specific marker of mesothelial cells; it is the only marker that has can distinguish between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in a panel of markers for distinguishing mesothelioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).
Specificity: A synthetic peptide corresponding to residues in human Calretinin was used as an immunogen.
Storage Condition and Buffer: Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
IHC: 1:100 - 250
ICC: 1:100 - 250
1. Plattelli A et al. J Endocl 29(6):394-396, 2003. 2. Ordonez NG et al. Hum Pathol. 35(6):697-710, 2004. 3. Ordonez NG et al. Hum Pathol. 30(3):313-23. 1999.
*For Mouse, this antibody is recommended for WB only
Rat cross reactivity tested by western blot only
Fig 1. Western blot analysis on (A) Fetal Brain (B) Mouse brain and (C) Rat brain lysates using anti-Calretinin RabMAb (cat. #2624-1).
Fig 2. Immunonohistochemical analysis of paraffin-embedded human brain tissue using anti-Calretinin RabMAb (cat. #2624-1).
Fig 3. Immunonohistochemical analysis of paraffin-embedded human mesothelioma tissue using anti-Calretinin RabMAb (cat. #2624-1).