Rabbit Monoclonal Antibody | Product Data Sheet
|Quantity: 100ul||Lot #: Please refer to vial|
|Clone ID: EP1938Y||Molecular Wt.: 25 kDa|
|Species Cross-reactivity* +Human-Mouse-Rat||UniProt ID: Q9Y2Q3|
Background: Glutathione transferases (GSTs) are a superfamily of enzymes that play a vital functional role in the cellular detoxification process. They catalyze the conjugation of the thiol group of glutathione (GSH) to the electrophilic groups of a wide range of hydrophobic substrates, leading to an easier removal of the latter from the cells. The kappa class is the least studied one among various classes within the superfamily. The TRX-like domain of hGSTK shares 19% sequence identity and structure similarity with human theta class GST, suggesting that the kappa class of GST is more closely related to the theta class enzyme within the GST superfamily (1). Western blotting analysis of mouse tissue homogenates demonstrated that mGSTK1 is expressed at relatively high levels in liver and stomach. Moderate expression was observed in kidney, heart, large intestine, testis, and lung, whereas sparse or essentially no mGSTK1 protein was detected in small intestine, brain, spleen, and skeletal muscle. Immunohistochemical (IHC) analysis for mGSTK1 revealed granular staining of hepatocytes throughout the liver, consistent with organelle staining. IHC analysis of murine kidney localized GSTK1 to the straight portion of the proximal convoluted tubule (pars recta) (2).
Specificity: A synthetic peptide corresponding to residues near the C-terminus of human Gstk1 was used as an immunogen.
Storage Condition and Buffer: Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
WB: 1:1,000 - 10,000
IHC: 1:100 - 250
ICC: 1:100 - 250
1. Li J, et al. Protein Sci, 14(9):2361-9, 2005
2. Thomson RE, et al. J Histochem Cyto 52(5):653-62, 2004
*Cross reactivity determined by western blot only.
Fig 1. A. Western blot analysis on fetal liver lysate using anti-Gstk1 RabMAb (cat. # 2050-1).
Fig 2. B. Immunohistochemical staining of paraffin-embedded human liver using anti-Gstk1 RabMAb (cat. #2050-1).