Background: Antigen-specific cell contacts in the immune system are strengthened by antigen-nonspecific interactions, mediated in part by lymphocyte-function associated (LFA) antigens. Recently, ICAM-1 (intercellular adhesion molecule-1) has been defined as a ligand for LFA-1. Monoclonal antibodies to ICAM-1 block T lymphocyte adhesion to fibroblasts and endothelial cells and disrupt the interaction between cytotoxic T cells and target cells. ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines (1). The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. However, ICAM-1 is also used as a receptor by the major group of human rhinoviruses and is a catalyst for the subsequent viral uncoating during cell entry (2). Monoclonal antibodies recognize a 95 kDa cell surface glycoprotein the major human rhinovirus receptor, ICAM-1 (3).

Specificity: A synthetic peptide corresponding to residues near the N-terminus of human ICAM-1 was used as an immunogen.

Storage Condition and Buffer: Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Recommended Dilutions:
WB: 1:1000 - 2000
IHC: 1:50 - 100
ICC: 1:100 - 250

Background References:
1. Simmons D, et al. Nature 331(6157):624-7, 1988.
2. Bella J, et al. Proc Natl Acad Sci USA 95(8)4140-5, 1998.
3. Greve JM, et al. Cell 56(5):839-47, 1989.

*Cross reactivity determined by western blot only.