Clone ID: SP8
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 4204-1
Size: 100ul
Price: $ 205
Recommended Applications: IHC
Species Reactivity: Human
Mol. Weight: 220 kDa
UniProt ID: Q9UM73
Alternative names: Anaplastic lymphoma kinase, CD246 antigen
Description: ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. The 2;5 chromosomal translocation occurs in most anaplastic large-cell non-Hodgkin's lymphomas arising from activated T lymphocytes. This rearrangement was shown to fuse the NPM nucleolar phosphoprotein gene on chromosome 5q35 to a previously unidentified protein tyrosine kinase gene, ALK, on chromosome 2p23 (1). In the predicted hybrid protein, the amino terminus of NPM is linked to the catalytic domain of ALK. Immunoblotting with anti-ALK antibody shows that ALK is highly expressed in the neonatal brain (2). Also expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. Unscheduled expression of the truncated ALK may contribute to malignant transformation in these lymphomas (3). Specificity: Recombinant protein corresponding to a region, which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript.
Recommended Dilutions:
IHC: 1:100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Immunohistochemical staining of paraffin-embedded human anaplastic lymphoma stained with anti-Alk/p80 RabMAb (Cat. #4204-1) |
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Description References:
| 1. |
Morris SW, et al. Science 263(5151):1281-4, 1994. |
| 2. |
Iwahara T, et al. Oncogene 14(4):439-49, 1997. |
| 3. |
Benharroch D, et al. Blood 91(6): 2076-84, 1998. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
