Clone ID: SP1
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 4200-1
Size: 100ul
Price: $ 205
Recommended Applications: WB, IHC, IP
Species Reactivity: Human, Mouse
Mol. Weight: 66 kDa
UniProt ID: P03372
Alternative names: ER, Estradiol receptor, ER-alpha
Description: Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation both function to regulate the transcriptional activation of ER alpha (2). Particularly, phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity (3). Specificity: A synthetic peptide derived from C-terminal of human estrogen receptor.
Recommended Dilutions:
WB: 1:1000
IHC: 1:100
IP: 1:10
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A. Western blotting assay using MCF-7 cell lysate with Rabbit anti-ER monoclonal antibody (cat# 4200-1), 1:1000 dilution |
B. Immunohistochemical staining of paraffin-embedded Human breast carcinoma stained with anti-ERRabMAb (Cat. #4200-1) |
Description References:
| 1. |
Pakdel, F., et al. Identification of charged residues in an N- terminal portion of the hormone-binding domain of the human estrogen receptor important in transcriptional activity of the receptor. Mol. Endocrinol. 7:1408 |
| 2. |
Sheeler, C.Q., et al. Mutation of serines 104, 106, and 118 inhibits dimerization of the human estrogen receptor in yeast. Endocr. Res. 29: 237 |
| 3. |
Rogatsky, I., et al. Potentiation of human estrogen receptor alpha transcriptional activation through phosphorylation of serines 104 and 106 by the cyclin A-CDK2 complex. J. Biol. Chem. 274: 22296 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
