Clone ID: EPR2751Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2421-1
Size: 100ul
Price: $ 250
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| A.Western blot analysis on human heart cell lysate using anti-Alpha B Crystallin RabMAb (cat. # 2421-1), dilution 1:2000. |
B. Immunohistochemical analysis of paraffin-embedded human muscle using anti- Alpha B Crystallin RabMAb (cat. #2421-1). |
Recommended Applications: WB, IHC, ICC
Species Reactivity: Human
Mol. Weight: 22 kDa
UniProt ID: P02511
Alternative names: Alpha(B)-crystallin, Rosenthal fiber component, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27
Description: Alpha B Crystallin, first identified as a structural component of the vertebrate eye lens, is expressed at high levels in lens and at lower levels in a number of other tissues, most notably cardiac and skeletal muscle, kidney, and brain (1). It is a major protein component of Rosenthal fibers (RF), which massively accumulate in the brains of patients suffering from Alexander's disease (2). The major phosphorylation site of Alpha B Crystallin in brains of patients with Alexander's disease or Alzheimer's disease as well as in aged controls is Serine 59 (3). Racemization, isomerization, and oxidation of Alpha B Crystallin are said to occur spontaneously in the aging process (4). Specificity: A synthetic peptide corresponding to residues in human Alpha B Crystallin was used as an immunogen.
Recommended Dilutions:
WB: 1:500 – 2000
IHC: 1:100 – 250
ICC: 1:100 – 250
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Dubin RA, et al. Genomics 7(4):594-601, 1990 |
| 2. |
Iwaki A, et al. Neurosci Lett 140(1):89-92, 1992 |
| 3. |
Kato K, et al. J Neurochem 76(3):730-6, 2001 |
| 4. |
Fujii N, et al. Biochim Biophys Acta 204(2):157-63, 1994 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
