Clone ID: EPR729(2)Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2411-1
Size: 100
Price: $ 285
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| A. Western blot analysis on (A) SH-SY5Y (B) human brain (C) mouse brain (D) rat brain lysate using anti-CaMKI RabMAb (cat. # 2331-1), dilution 1:5000. |
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Recommended Applications: WB
Species Reactivity: Human, Mouse
Mol. Weight: 17 kDa
UniProt ID: Q13541
Alternative names: eIF4E-binding protein 1, 4E-BP1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I
Description: 4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eIF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP1. Non-phosphorylated 4E-BP1 will bind strongly to eIF4E while, the phosphorylated form will no (2). Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 70 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3). It has been reported previously that phosphorylation of 4E-BP1 on Thr 37 and Thr 46 is relatively insensitive to serum deprivation and rapamycin treatment, and that phosphorylation of these residues is required for the subsequent phosphorylation of a set of unidentified serum-responsive sites (4) Specificity: A phospho specific peptide corresponding to residues surrounding threonine 37 was used as an immunogen. This antibody detects 4E-BP1 phosophorylated at threonine 37.
Recommended Dilutions:
WB: 1:1000 - 2000
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Pause, A., et al. 1994. Nature 371: 762-767 |
| 2. |
Gingras, A.-C., et al. Genes & Dev. 12: 502-513, 1998 |
| 3. |
Iritani, BM et al. (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 13180 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
