Clone ID: EPR1923Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2306-1
Size: 100
Price: $ 250
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| A. Western blot analysis on (A) C6 (B) MCF-7 (C) HeLa and (D) A431 cell lysate using anti-Cortactin RabMAb (cat. # 2306-1), dilution 1:5000. |
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Recommended Applications: WB, ICC, FC
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 80/85 kDa
UniProt ID: Q14247
Alternative names: Amplaxin, Oncogene EMS1
Description: Immunocytochemistry reveals that in epithelial cells, the human cortactin protein is localized mainly in the cytoplasm and, to a very low extent, in protruding leading lamellae of the cell. However, in carcinoma cells that constitutively overexpress cortactin accumulates in the podosome-like adherens junctions associated with the cell-substratum contact sites. Overexpression and concomitant accumulation of the Cortactin protein in the cell-substratum contact sites might, therefore, contribute to the invasive potential of these tumor cells (1). In cells derived from breast carcinomas and squamous carcinomas of the head and neck, DNA amplification of a particular tyrosine kinase substrate region results in overexpression of cortactin. Overexpression is accompanied by a partial redistribution of cortactin from the cytoplasm into cell-matrix contact sites (2). Specificity: A synthetic peptide corresponding to residues near the C-terminus of human cortactin was used as an immunogen.
Recommended Dilutions:
WB: 1:5000
ICC: 1:100
Flow Cytometry: 1:30
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Schuuring E, et al. Mol Cell Biol. 13(5):2891-98, 1993 |
| 2. |
Van Damme H, et al. J Biol Chem 272(11):7374-80, 1997 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
