Clone ID: EPR1172Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2305-1
Size: 100
Price: $ 250
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| A. Western blot analysis on 293T cell lysate using anti-4-1BBL RabMAb (cat. # 2305-1), dilution 1:2000. |
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Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human
Mol. Weight: 26 kDa
UniProt ID: P41273
Alternative names: 4-1BB ligand, 4-1BBL
Description: 4-1BB was originally described as a cDNA expressed by activated murine T cells and subsequently demonstrated to encode a member of the tumor necrosis factor receptor family of integral membrane proteins. Recently, a murine ligand for 4-1BB (mu4-1BB-L) was cloned and demonstrated to be a member of an emerging family of ligands with structural homology to tumor necrosis factor. Both monoclonal antibody to hu4-1BB and cells transfected with hu4-1BB-L induced a strong proliferative response in mitogen co-stimulated primary T cells. In contrast, ligation of 4-1BB on T cell clones enhanced activation-induced cell death when triggered by engagement of the TCR/CD3 complex. (1). To reject tumors, T cells must overcome poor tumor immunogenicity and an adverse tumor microenvironment. It has been shown that primary human T cells expressing CD80 and 4-1BBL vigorously respond to tumor cells lacking costimulatory ligands and provoke potent rejection of large, systemic tumors in immunodeficient mice (2). Specificity: A synthetic peptide corresponding to residues near the N-terminus of human 4-1BBL was used as an immunogen.
Recommended Dilutions:
WB: 1:500
IHC: 1:100
ICC: 1:100
IP: 1:40
Protocols for using Epitomics Rabbit Monoclonal Antibodies
References:
| 1. |
Alderson MR, et al. Eur J Immunol 24(9):2219-27, 1994 |
| 2. |
Steohan MT, et al. Nat Med 13(12):1440-9, 2007 |
Orders:
877-772-2622 (US and Canada)
650-443-2276 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
