Clone ID: EPR2540Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2304-1
Size: 100
Price: $ 250
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| A. Western blot analysis on (A) TF-1 (B) U937 cell lysate and (C) Jurkat cell lysate using anti-WASP RabMAb (cat. # 2304-1), dilution 1:2000. |
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Recommended Applications: WB, ICC, FC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 60 kDa
UniProt ID: P42768
Alternative names: WASp
Description: The Wiskott-Aldrich syndrome (WAS) is an X-chromosome-linked recessive disease characterized by eczema, thrombocytopenia, and immunodeficiency (1). WAS is an immunodeficiency disorder with the most severe pathology in the T lymphocytes and platelets. The disease arises from mutations in the gene encoding the WAS protein (WASP). T lymphocytes of affected males with WAS exhibit a severe disturbance of the actin cytoskeleton, suggesting that the WASP could regulate its organization. Data suggest that the WASP might function as a signal transduction adaptor downstream of Cdc42, and in affected males, the cytoskeletal abnormalities may result from a defect in Cdc42 signaling (2). WASP is a key regulator of the Arp2/3 complex and the actin cytoskeleton in hematopoietic cells. WASP is capable of forming an auto-inhibited conformation, which can be disrupted by binding of Cdc42 and phosphatidylinositol 4,5-bisphosphate, leading to its activation. Stimulation of the collagen receptor on platelets and crosslinking the B-cell receptor induce tyrosine phosphorylation of WASP (3). Specificity: A synthetic peptide corresponding to residues near the N-terminus of human WASP was used as an immunogen.
Recommended Dilutions:
WB: 1:1000 - 2000
IHC: 1:100
Flow Cytometry: 1:30
IP: 1:30
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Smith FJ, et al. Biochem Biophys Res Commun. 297(4):818-27, 2002. |
| 2. |
Adley BP, et al. Anal Quant Cytol Histol 28(4):228-36, 2006 |
| 3. |
Coons SW, et al. Endocr Pathol 16(3):201-10, 2005 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
