Clone ID: EPR1619Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2303-1
Size: 100
Price: $ 250
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| A. Western blot analysis on (A) Hela cell lysate and (B) T47D using anti-CK-7 RabMAb cat. # 2303-1), dilution 1:10,000. |
B. Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using anti-CK-7 RabMAb (cat.#2303-1) |
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| C. Immunofluorescent staining of HeLa cells using anti-CK-7 RabMAb (cat.#2303-1). |
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Recommended Applications: WB, IHC, ICC, FC, IP
Species Reactivity: Human
Mol. Weight: 55 kDa
UniProt ID: P08729
Alternative names: Cytokeratin-7, CK-7, Keratin-7, K7, Sarcolectin
Description: Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Keratin 7 (CK7) is expressed in a wide range of epithelial structures in humans. The expression pattern in mice is similar to the reported human distribution, with substantial expression observed in lung, bladder, mesothelium, hair follicle, and ductal structures (1). Protein expression levels of CK7 appear significantly higher in chromophobe RCC compared to oncocytoma (p < 0.001). Studies provide further evidence that CK7 and parvalbumin immunostains may be useful in differentiating oncocytoma from chromophobe RCC in problematic cases (2). Also, the pattern of immunohistochemical expression of CK 7 along with CK 20 is commonly used to assess possible primary sites of metastatic carcinomas (3). Specificity: A synthetic peptide corresponding to residues near the N-term of human CK-7 was used as an immunogen.
Recommended Dilutions:
WB: 1:5000
IHC: 1:100
ICC: 1:100
Flow Cytometry: 1:30
IP: 1:30
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Smith FJ, et al. Biochem Biophys Res Commun. 297(4):818-27, 2002 |
| 2. |
Adley BP, et al. Anal Quant Cytol Histol 28(4):228-36, 2006 |
| 3. |
Coons SW, et al. Endocr Pathol 16(3):201-10, 2005. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
