Clone ID: EP2270Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2295-1
Size: 100
Price: $ 285
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| A. Western blot analysis on A431 cell lysates using anti-Phospho-Her4/ErbB-4 (pY1162) RabMAb (cat. #2295-1), 1:500 dilution. Cells were either (A) untreated (B) treated with EGF. |
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-HER-4 RabMAb
(cat. # 2295-1). |
Recommended Applications: WB, IHC, IP
Species Reactivity: Human
Mol. Weight: 180 kDa
UniProt ID: Q15303
Alternative names: p180erbB4, Tyrosine kinase-type cell surface receptor HER4
Description: HER4 / ErbB4 is a receptor tyrosine kinase that belongs to the human epidermal growth factor receptor (EGFR) family. ErbB4/HER4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum (1). ErbB4/HER4 is activated by neuregulins (NRG), betacellulin (BTC), and heparin-binding EGF-like growth factor (2). Neuregulins regulate the expression of ligand- and voltage-gated channels in neurons and skeletal muscle by the activation of ErbB 1-4. The erbB4/HER4 receptor is unique among these receptors because its C-terminal tail binds to a protein motif known as the PDZ domain (3). Specificity: A phospho specific peptide corresponding to residues surrounding tyrosine 1162 of human Her4/ErbB-4 was used as an immunogen. This antibody detects Her4/ErbB-4 phosphorylated on tyrosine 1162.
Recommended Dilutions:
WB: 1:25,000
IHC: 1:100
IP: 1:30
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Plowman, G.D., et al. Proc Natl Acad Sci U S A. 90: 1746 |
| 2. |
Elenius, K., et al. J Biol Chem. 272: 26761 |
| 3. |
Garcia, R.A., et al. Proc Natl Acad Sci U S A. 97: 3596 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
