Clone ID: EP1932Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2293-1
Size: 100
Price: $ 250
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| A. Western blot analysis on HepG2 cell lysate using anti-Aldh1a1 RabMAb (cat. # 2293-1), dilution 1:1000. |
B. Immunohistochemical analysis of paraffin-embedded human liver using anti-Aldh1a1 RabMAb (cat. #2293-1). |
Recommended Applications: WB, IHC
Species Reactivity: Human
Mol. Weight: 55 kDa
UniProt ID: P00325
Alternative names: Alcohol dehydrogenase subunit beta
Description: Acetaldehyde dehydrogenase (ALDH) is the next enzyme after alcohol dehydrogenase in the major pathways of alcohol metabolism. Usual human livers contain two major ALDH isozymes, i.e., cytosolic ALDH1 and mitochondrial ALDH2(1). Human red cell aldehyde dehydrogenase (ALDH) resembles the liver cytosolic isozyme in numerous physicochemical properties. Thus, the erythrocyte enzyme, by virtue of its chemical and structural identity with the liver cytosolic enzyme, may serve as a suitable peripheral enzyme model to understand the cause and mechanism of alcohol abuse-related changes in liver cytosolic ALDH that has been found to be reduced in alcoholics (2). ALDH1A1 is known to catalyze the oxidation of retinaldehyde to retinoic acid. Overexpression of human ALDH1A1 in HEK cells led to a more than 20-fold increase in 3-deoxyglucosone dehydrogenase activity (3). Specificity: A synthetic peptide corresponding to residues in human ALdh1a1 was used as an immunogen.
Recommended Dilutions:
WB: 1:1000
IHC: 1:100 - 250
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Yoshida A, et al. Alcohol 2(1):103-6, 1985 |
| 2. |
Agarwal DP, et al. Enzyme 42(1):47-52, 1989 |
| 3. |
Collard F, et al. Biochimie 89(3):369-73, 2007 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
