Clone ID: EP1405Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2263-1
Size: 100
Price: $ 285
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| A. Western blot analysis on Raji cell lysates using anti-Phospho- PLCγII (pY1197) RabMAb (cat. #2263-1), 1:20,000 dilution. Cells were either (A) untreated (B) treated with pervanadate. |
B. |
Recommended Applications: WB, ICC, IP
Species Reactivity: Human
Mol. Weight: 150 kDa
UniProt ID: EP1405Y
Alternative names:
Description: Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups. The phospholipase C gamma-2 (PLCγII) is an enzyme that plays a crucial role in intracellular signal transduction pathways. This enzyme is important because of its role in the generation of second messengers following the hydrolysis of phosphatidylinositol 4,5-bisphosphate (1). PLCγII has been implicated in collagen-induced signal transduction in platelets and antigen-dependent signaling in B-lymphocytes. It has been suggested that tyrosine kinases activate PLCγII (2). Btk-deficient B cells exhibited a marked reduction in phosphorylation of the Src homology 2 (SH2)-SH3 linker region sites, Tyr(753) and Tyr(759). Phosphorylation of both sites was restored by expression of Tec, but not Syk, family kinases. In contrast, phosphorylation of the PLCgamma2 carboxyl-terminal sites, Tyr(1197) and Tyr(1217), was unaffected by the absence of functional Btk (3). Specificity: A phospho specific peptide corresponding to residues surrounding tyrosine 1197 of human PLCγII was used as an immunogen. This antibody detects PLCγII phosphorylated on tyrosine 1197.
Recommended Dilutions:
WB: 1:2,000
ICC: 1:100
IP: 1:40
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Argeson AC, et al. Genomics 25(1):29-35, 1995. |
| 2. |
Ozdener F, et al. Mol Pharmacol 62(3):672-9, 2002. |
| 3. |
Humprhries LA, et al. J Biol Chem 279(36):37651-61, 2004 |
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Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
