Clone ID: EP2393Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2262-1
Size: 100
Price: $ 285
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| A. Western blot analysis on T47D cell lysates using anti-Phospho-p53 (pS33) RabMAb (cat. #2262-1), 1:2000 dilution. Cells were either (A) untreated (B) treated with etoposide. |
B. Immunofluorescent staining of A431 cells using anti-Phospho-p53 RabMAb (cat. # 2262-1). |
Recommended Applications: WB, ICC
Species Reactivity: Human
Mol. Weight: 53 kDa
UniProt ID: P04637
Alternative names: Tumor suppressor p53, Phosphoprotein p53, Antigen NY-CO-13
Description: p53 acts as both a tumor-suppressor and transcription factor that, upon activation by DNA damage and other cellular stress signals, leads to the transcription of genes triggering cell-cycle arrest, apoptosis, and DNA repair (1,2). The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers (3). p53 is frequently mutated or inactivated in about 60% of cancers (4,5). Cyclin dependent kinase 9, whose well-known substrate is RNA polymerase II, can also phosphorylate p53, more specifically, Ser33 on the N-terminus and, Ser315 and Ser392 on the C-terminus (6). Specificity: A phospho specific peptide corresponding to residues surrounding serine 33 of human p53 was used as an immunogen. This antibody detects p53 phosphorlyated at serine 53.
Recommended Dilutions:
WB: 1:1000
ICC: 1:100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Levine, A.J. et al. Cell 88: 323 |
| 2. |
Lakin, N.D. et al. Oncogene 18: 7644 |
| 3. |
Vogelstein, B. et al. Nature 348: 681 |
| 4. |
Hoolstein, M., et al. Science 253: 49 |
| 5. |
Harris C.C. Science 262: 1980 |
| 6. |
Radhakrishnan SK, et al. Cell Cycle 5(5):519-21, 2006 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
