Clone ID: EPR2241IHC
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2261-1
Size: 100ul
Price: $ 250
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| A. Western blot analysis on MCF-7 cell lysate using anti-Cyclin D1 RabMAb (cat. # 2261-1), dilution 1:50,000. |
A. Immunohistochemical analysis of paraffin-embedded human mantle cell lymphoma using anti-Cyclin D1 RabMAb (cat. #2261-1). |
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 36 kDa kDa
UniProt ID: P24385
Alternative names:
Description: Cyclin D1 belongs to the Cyclin D family. Cyclin D1 is required for the cell cycle G1/S transition (1). Cyclin D1 forms a serine/threonine kinase holoenzyme complex with and functions as a regulatory subunit of CDK4 or CDK6. Cyclin D1 also phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1/S phase (2-3). Amplification or overexpression of cyclin D1 plays a pivotal role in the development of various human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer (4). Over-expression of cyclin D1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis (5). Cyclin D1 also associates with, and regulates the activity of transcription factors, coactivators and corepressors that govern histone acetylation and chromatin remodeling proteins (6-7) Specificity: A synthetic peptide corresponding to residues near the C-terminus of human Cyclin D1 was used as an immunogen.
Recommended Dilutions:
WB: 1:10,000
IHC: 1:100
ICC: 1:100
IP: 1:40
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Inaba et al. Genomics 13:565-574, 1992 |
| 2. |
Sherr CJ Canc Res 60:3689 |
| 3. |
Baldin et al. Genes Dev 7:812 |
| 4. |
Bala S, Peltomaki P Cancer Res 61:6042 |
| 5. |
Motokura T, Arnold A Genes Chrom. Canc. 7:89 |
| 6. |
Wang et al. Cancer Treat Res 119:217 |
| 7. |
Neumeister et al. Mol Biol Cell 14:2005 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
