Clone ID: EP2261Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2260-1
Size: 100
Price: $ 285
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| A. Western blot analysis on 293T cell lysates using anti-Phospho-eIF4B (pS504) RabMAb (cat. #2260-1), 1:50,000 dilution. Cells were either (A) untreated (B) treated with AP. |
B. |
Recommended Applications: WB
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 80 kDa
UniProt ID: P23588
Alternative names: eIF-4B
Description: Eukaryotic protein synthesis initiation factor 4B (eIF-4B) is an 80,000 dalton polypeptide which is essential for the binding of mRNA to ribosomes. The amino-terminal domain of eIF-4B contains a consensus RNA binding domain present in a number of other RNA binding proteins (1). It contains a ribonucleoprotein consensus sequence (RNP-CS)/RNA recognition motif (RRM). Deletions which remove this region abolish the ability of eIF-4B to cooperate with eIF-4A in RNA binding and the ability to stimulate the helicase activity of eIF-4A. Results indicate that the carboxy-terminal RNA-binding region of eIF-4B is essential for eIF-4B function and is distinct from the RNP-CS/RRM (2). eIF4F binds to the mRNA cap structure and, in combination with eIF4B, is believed to unwind the secondary structure in the 5' untranslated region to facilitate ribosome binding. It has been suggested that eIF4B participates in mRNA-ribosome binding by acting as an intermediary between the mRNA and eIF3, via a direct interaction with the p170 subunit of eIF3 (3). Specificity: A phospho specific peptide corresponding to residues surrounding S504 of human eIF4B was used as an immunogen. This antibody detects eIF4B phosphorylated at serine 504.
Recommended Dilutions:
WB: 1:25,000
ICC: 1:100 -250
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Milburn SC, et al. EMBO J, 9(9):2783-90, 1990 |
| 2. |
Methot N, et al. Mol Cell Biol 14(4):2307-16, 1994 |
| 3. |
Methot N, et al. Mol Cell Biol 16(10):5328-34, 1996 |
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877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
