Clone ID: EP1516Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2258-1
Size: 100
Price: $ 285
|
 |
| A. Western blot analysis on T47D cell lysates using anti-Phospho-PR (pS190) RabMAb (cat. #2258-1), 1:10,000 dilution. Cells were either (A) untreated (B) treated with progesterone. |
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Phospho-PR RabMAb. (cat. # 2258-1). |
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human
Mol. Weight: 90/118 kDa
UniProt ID: P06401
Alternative names: PR, Nuclear receptor subfamily 3 group C member 3
Description: The human progesterone receptor (PR), is a ligand-activated transcription factor and is a member of the steroid receptor family (1). PR exists in humans as two isoforms; PR-A (94 kDa) which lacks the first 164 amino acids of PR-B and PR-B (114 kDa)(2-3). While the two forms of PR have similar DNA- and ligand-binding affinities they have opposite transcriptional activities. PR-B functions as an activator of progesterone-responsive genes, while PR-A functions as a strong transdominant repressor of PR-B (4). Human progesterone receptor (PR) is phosphorylated on multiple serine residues (at least seven sites) in a manner that involves distinct groups of sites coordinately regulated by hormone and different kinases. As detected by Western blot of T47D cells treated with hormone for different times, hormone-dependent down-regulation of total PR and the Ser 190 phosphorylation site occurred in parallel, whereas the Ser 294 phosphorylation site was down-regulated more rapidly (5) Specificity: A phospho specific peptide corresponding to residues surrounding serine 190 of human PR was used as an immunogen. This antibody detects PR phophorylated at serine 190.
Recommended Dilutions:
WB: 1:5000
IHC: 1:100
ICC: 1:100
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Evans, R. M. 1988. Science 240:889-895 |
| 2. |
Paloma H. et al. May 2000, p. 3102-3115, Vol. 20, No. 9 |
| 3. |
Giangrande, P. et al. 1997. J. Biol. Chem. 272: 32889-32900 |
| 4. |
B. Mulac-Jericevic et al. Reproduction, 2004; 128(2): 139 |
| 5. |
Clemm DL, et al. Mol Edocrinol 14(1):52-65, 2000 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
