Clone ID: EP1841Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2257-1
Size: 100
Price: $ 250
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| A. Western blot analysis on (A) HeLa cell lysate (B) HepG2 cell lysate (C) Jurkat cell lysate using anti-PTP1B RabMAb (cat. # 2257-1), dilution 1:2000. |
B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-PTP1B RabMAb. (cat. #2257). |
Recommended Applications: WB, IHC, ICC, FC
Species Reactivity: Human
Mol. Weight: 50 kDa
UniProt ID: P18031
Alternative names: Protein-tyrosine phosphatase 1B, PTP-1B
Description: The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3). Specificity: A synthetic peptide corresponding to residues surrounding serine 386 of human PTP1B was used as an immunogen. This antibody detects both phosphorylated and nonphosphorylated PTP1B.
Recommended Dilutions:
WB: 1:1000
IHC: 1:50
ICC: 1:50
Flow Cytometry: 1:20
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Flint AJ, et al. EMBO J 12(5):1937-46, 1993 |
| 2. |
Frangioni JV, et al. Cell 68(3):545-60, 1992 |
| 3. |
Ravichandran LV, et al Mol Endocrinol 5(10):1768-80, 2001. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
