Clone ID: EP928Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2256-1
Size: 100
Price: $ 250
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| A. Western blot analysis on human brain lysate using anti-GAP43 RabMAb (cat. # 2256-1), dilution 1:100,000. |
B. |
Recommended Applications: WB, ICC
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 48 kDa
UniProt ID: P17677
Alternative names: Axonal membrane protein GAP-43, Growth-associated protein 43, PP46, Neural phosphoprotein B-50
Description: Growth associated protein GAP43 (B-50, F1, neuromodulin) is a phosphoprotein of the presynaptic membrane involved in synaptic reorganization and neural development. Phospholyated at Ser41 by protein kinase C (PKC), phospholyated GAP43 has been correlated to release of neurotransmitters in mature nervous system (1). Also, GAP43 plays an essential role in the maintenance mechanism of long-term potentiation (LTP) (2). These suggest GAP43 to play a role in synaptic plasticity. For neurons in development or regeneration, GAP43 is a major component of the growth cone, which enables elongation and navigation of developing axons (3). During periods of low activity in neurons, Calmodulin binds to GAP43 when Ca2+ is low, suggesting Calmodulin as a negative regulator of GAP43 (4). Specificity: A synthetic peptide corresponding to residues surrounding serine 41 of human GAP43 was used as an immunogen. This antibody detects both phosphorylated and nonphosphorylated GAP43.
Recommended Dilutions:
WB: 1:50,000
ICC: 1:100 - 250
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
McIntosh H. et al. Visual Neuroscience. 2: 583-591, 1989. |
| 2. |
Gianotti C, et al. Neuron 8:843-8, 1992. |
| 3. |
Skene JH, et al. Science 233:783-786, 1986. |
| 4. |
Neve RL, et al. J Neuro 18:7757-7767, 1998. |
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orders@epitomics.com
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Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
