Clone ID: EP1902Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2252-1
Size: 100
Price: $ 285
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| A. Western blot analysis on Jurkat cell lysates using anti-Phospho- PKCβII (pS660) RabMAb (cat. #2252-1), 1:100,000 dilution. Cells were either (A) untreated (B) treated with AP. |
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti- PKCβII RabMAb. (cat. # 2252-1). |
Recommended Applications: WB, IHC, ICC
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 80 kDa
UniProt ID: P05771
Alternative names: PKC-beta, PKC-B
Description: PKCβII is a member of the serine/threonine protein kinase C (PKC) family (1) that includes 10 closely related isozymes classified as the conventional, the novel and the atypical. The conventional PKCβII is implicated in diverse cellular process including secretion, proliferation, differentiation, apoptosis, permeability, migration & hypertrophy (2-3). PKCβII structure consists of a C-terminal catalytic region, containing an activator-binding domain and a Ca2+-binding domain, and a regulatory domain. It has been shown that PKCβII must first be processed by three distinct phosphorylations before it is competent to respond to second messengers. Specifically, the threonine at position 500 (T500) on the activation loop, and T641 and S660 on the carboxyl terminus of protein kinase C beta II are phosphorylated in vivo. T500 and S660 are selectively dephosphorylated in vitro by protein phosphatase 2A to yield an enzyme that is still capable of lipid-dependent activation, whereas all three residues are dephosphorylated by protein phosphatase 1 to yield an inactive enzyme. (4). Specificity: A phospho specific peptide corresponding to residues surrounding serine 660 of human PKCβII was used as an immunogen. This antibody detects PKCβII phosphorylated at serine 660.
Recommended Dilutions:
WB: 1:10,000
IHC: 1:100
ICC: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
References:
| 1. |
Coussens L, et al (986) Science;233(4766):859-66. |
| 2. |
Mellor, H., et al (1998) Biochem. J. 332, 281-292 |
| 3. |
Nishizuka, Y. (1995) FASEB J. 9, 484-496 |
| 4. |
Keranen LM, et al. Curr Biol 5(12):1394-1403, 1995 |
Orders:
877-772-2622 (US and Canada)
650-443-2276 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
