Clone ID: EPR1977Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2251-1
Size: 100
Price: $ 250
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| A. Western blot analysis on (A) NIH/3T3 or (B) HeLa cell lysate using anti-GAPDH RabMAb (cat. # 2251-1), dilution 1:2000. |
B. Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-GAPDH RabMAb (cat. #2251-1). |
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 35 kDa
UniProt ID: P04406
Alternative names:
Description: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was considered a classical glycolytic protein involved exclusively in cytosolic energy production. However, recent evidence suggests that it is a multifunctional protein displaying diverse activities distinct from its conventional metabolic role. These new roles for GAPDH may be dependent on its subcellular localization, oligomeric state or on the proliferative state of the cell. Results suggest that human cells contain significant intracellular levels of enzymatically inactive GAPDH which is age-independent. The possibility is considered that the functional diversity of GAPDH may be mediated either by posttranslational alteration or by subcellular protein:protein and/or protein:nucleic acid interactions (1). GAPDH mRNA levels, protein, and enzymatic activity have been shown to increase increase in 3T3 cells after exposure to physiological concentrations of insulin (2). Specificity: A synthetic peptide corresponding to residues in human GAPDH was used as an immunogen.
Recommended Dilutions:
WB: 1:1000
IHC: 1:100
ICC: 1:50
IP: 1:40
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Mazzola JL, et al. BioChim Biophys Acta 20(1622):50-6, 2003 |
| 2. |
Ercolani L et al. J Biol Chem, 263(3): 15335-41, 1988 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
