Clone ID: EPR654(2)A
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2250-1
Size: 100
Price: $ 285
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| A. Western blot analysis on 293T cell lysates using anti-Phospho-4E-BP1 (pT70) RabMAb (cat. #2250-1), 1:500 dilution. Cells were either (A) untreated (B) treated with FBS. |
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Recommended Applications: WB, IHC
Species Reactivity: Human
Mol. Weight: 17 kDa
UniProt ID: Q13541
Alternative names: 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I
Description: 4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 70 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4) Specificity: A phospho specific peptide corresponding to residues surrounding threonine 70 was used as an immunogen. This antibody detects 4E-BP1 phosophorylated at threonine 70.
Recommended Dilutions:
WB: 1:500
IHC: 1:100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Pause, A., et al. 1994. Nature 371: 762-767 |
| 2. |
Gingras, A.-C., et al. Genes & Dev. 12: 502-513, 1998 |
| 3. |
Iritani, BM et al. (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 13180 |
| 4. |
Trumpp, A. et al. Nature 414, 768 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
