Clone ID: EP206Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2249-1
Size: 100
Price: $ 250
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| A. Western blot analysis on (A) human brain lysate (B) rat brain lysate and (C) HeLa cell lysate using anti-FAK2 RabMAb (cat. # 2249-1), dilution 1:2000. |
B. Immunohistochemical analysis of paraffin-embedded human brain using anti-FAK2 RabMAb (cat. #2249-1). |
Recommended Applications: WB, IHC, IP
Species Reactivity: Human, Rat
Mol. Weight: 116 kDa
UniProt ID: Q14289
Alternative names: Focal adhesion kinase 2, FADK 2, Proline-rich tyrosine kinase 2, Cell adhesion kinase beta, CAK beta, Calcium-dependent tyrosine kinase, CADTK, Related adhesion focal tyrosine kinase, RAFTK
Description: Focal adhesion kinase 2 (FAK2), which is highly expressed in the central nervous system, is rapidly phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by protein kinase C activation. Activation of FAK2 leads to modulation of ion channel function and activation of the MAP kinase signalling pathway. FAK2 activation may provide a mechanism for a variety of short- and long-term calcium-dependent signalling events in the nervous system (1). FAK2 is activated by a variety of G-protein-coupled receptors and by extracellular signals that elevate intracellular Ca2+ concentration. Pap has been identified as a new Pyk2 binding protein. It has been demonstrated that Pap forms a stable complex with Pyk2 and that activation of Pyk2 leads to tyrosine phosphorylation of Pap in living cells. Immunofluorescence experiments demonstrate that Pap is localized in the Golgi apparatus and at the plasma membrane, where it is colocalized with Pyk2. Pap functions as a GAP for Arf and that Pyk2 may be involved in regulation of vesicular transport through its interaction with Pap (2). Specificity: A synthetic peptide corresponding to residues on the C-terminus of human FAK2 was used as an immunogen.
Recommended Dilutions:
WB: 1:1000
IHC: 1:100
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Lev S, et al. Nature 376(6543):737-45, 1995 |
| 2. |
Andreev J et al. Mol Cell Biol 19(3):2338-50, 1999 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
