Clone ID: EP913(2)Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2239-1
Size: 100
Price: $ 285
|
 |
| A. Western blot analysis on human brain lysates using anti-Phospho-E-Cadherin (pS838/840) RabMAb (cat. #2239-1), 1:1,000,000 dilution. Cells were either (A) untreated (B) treated with AP |
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-anti-E-Cadherin RabMAb. (cat. #2239-1) |
|
|
| C. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using Phospho-E-Cadherin RabMAb.(cat.#2239-1). |
|
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 135 kDa
UniProt ID: P12830
Alternative names: E-cadherin, Uvomorulin, Cadherin-1, CAM 120/80, CD324 antigen
Description: E-Cadherin, a member of the cadherin superfamily, is implicated as a key player in different cellular processes including development, morphology, polarity, migration and tissue integrity (1-2). E-cadherin is a glycoprotein with an extracellular domain that interacts with other E-cadherin molecules on adjacent cells, thereby establishing adhesion between epithelial cells. Established as a tumor and metastasis suppressor, E-cadherin expression is frequently down-regulated or extinguished in malignancy which strongly correlates with poor prognosis (3-4). Mutations in E-cadherin are found in gastric, breast, colorectal, thyroid and ovarian cancer (5). Specificity: A phospho-specific peptide corresponding to residues surrounding serine 838/840 of human E-cadherin was used as an immunogen. This antibody detects E-cadherin phosphorylated at serine 838 and 840
Recommended Dilutions:
WB: 1:100,000
IHC: 1:100
ICC: 1:250
IP: 1:40
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Mansouri, A.; Goodfellow, P. N.; Kemler, R. : Cytogenet. Cell Genet. 46: 655, 1987. |
| 2. |
Vleminckx K, Kemler R. Bioessays. 1999;21:211 |
| 3. |
Nollet, F., G. Berx, and F. van Roy. 1999. Mol. Cell Biol. Res. Comm. 2:77 |
| 4. |
Birchmeier, W., and J. Behrens. 1994. Biochim. Biophys. Acta. 1198:11 |
| 5. |
Thoreson MA, Reynolds AB. Differentiation. 2002 Dec;70(9-10):583-9 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
