Clone ID: EP1874Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2230-1
Size: 100
Price: $ 285
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| A. Western blot nalysis on HeLa cell lysates using anti-Phospho-Histone H3.3 (pS28 & S31) RabMAb (cat. #2230-1), 1:5000 dilution. Cells were either (A) untreated (B) treated with Hela+FBS +calyculin A |
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Recommended Applications: WB
Species Reactivity: Human
Mol. Weight: 17 kDa
UniProt ID: P84244
Alternative names: H3f3a, H3f3b
Description: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Various post-translational modifications, such as acetylation and methylation, of core histones serve as regulators of gene expression (2). Histone H3.3 is a variant histone which replaces conventional H3 in nucleosomes in active genes. It is predominant from of histone H3 in non-dividing cells and it is incorporated into chromatin independently of DNA synthesis. H3.3 is phosphorylated at serine 28 by MLTK isoform 1, RPS6KA5, and AURKB during mitosis. Also, phosphorylation at serine 31 on H3.3 is specific to regions bordering centromeres in metaphase chromosomes. Specificity: A synthetic phospho-peptide corresponding to residues surrounding serine 28 and serine 31 of human Histone H3.3 protein.
Recommended Dilutions:
WB: 1:2000
Protocols for using Epitomics Rabbit Monoclonal Antibodies
References:
| 1. |
Braunstein M, et al. Mol. Cell. Biol. 16:4349 |
| 2. |
Workman JL, et al. Annu. Rev. Biochem. 67: 545 |
| 3. |
Cheung, P. et al. Cell 103, 263 |
| 4. |
Xiao T, et al. Mol Cell Bio 25:637-651, 2005 |
| 5. |
Albig W, et al. Hum Genet 97:486-491. 1996 |
Orders:
877-772-2622 (US and Canada)
650-443-2276 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
